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棉花学报 ›› 2018, Vol. 30 ›› Issue (4): 308-315.doi: 10.11963/1002-7807.wxyaxt.20180723

• 研究与进展 • 上一篇    下一篇

基于SSR标记新疆陆地棉的DNA指纹图谱构建及遗传多样性分析

王欣怡1,李雪源2,龚照龙2,王俊铎2,范李萍1,郑巨云2,梁亚军2,郭江平2,买买提·莫明2,艾先涛2*   

  1. 1.新疆农业大学农学院/农业生物技术重点实验室,乌鲁木齐 830052;2.新疆农业科学院经济作物研究所,乌鲁木齐 830091
  • 收稿日期:2017-06-07 出版日期:2018-07-15 发布日期:2018-07-15
  • 通讯作者: yixiantao@sina.com
  • 基金资助:
    新疆自然科学基金项目(2017D01A45)

DNA Fingerprint Construction and Genetic Diversity Analysis Based on SSR Markers for Upland Cotton in Xinjiang

Wang Xinyi1, Li Xueyuan2, Gong Zhaolong2, Wang Junduo2, Fan Liping1, Zheng Juyun2, Liang Yajun2, Guo Jiangping2, Mamat Moming2, Ai Xiantao2*   

  1. 1. Key Laboratory of Agriculture Biological Technology / College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China; 2. Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2017-06-07 Online:2018-07-15 Published:2018-07-15

摘要: 【目的】利用Simple sequence repeats(SSR)标记构建新疆近40年间审定的120个陆地棉品种的DNA指纹图谱并进行遗传多样性分析。【方法】从586对候选引物中筛选得到78对多态性高、扩增稳定且均匀分布于棉花染色体上的引物,并用这78对引物构建120个陆地棉品种的DNA指纹图谱。【结果】78对核心引物在120个材料中检测到392个等位位点,其中多态性位点324个,多态性比率达82.7%。24个标记位点在17个品种上具有特征谱带。采用12对引物组合即可鉴定120个棉花品种。聚类分析显示,120个陆地棉品种遗传相似系数变化范围为0.50~0.96,平均为0.73,遗传相似系数偏高,表明新疆陆地棉品种的遗传基础较狭窄。【结论】引物组合法是构建DNA指纹图谱最有效的方法。遗传相似系数矩阵将120个陆地棉品种分为三大类群,与系谱来源较为吻合。

关键词: 新疆; 陆地棉; SSR分子标记; DNA指纹图谱; 遗传多样性

Abstract: [Objective] The aim of this study was to construct a DNA fingerprinting database of 120 upland cotton cultivars from Xinjiang and to analyze their genetic diversity based on SSR markers. [Methods] Seventy-eight evenly distributed SSR primer pairs with high polymorphism and good repeatability were successfully screened out from 586 candidates to construct the fingerprinting database. [Result] A total of 392 alleles from 120 varieties were screened using 78 pairs of core primers, 324 of which were polymorphic loci with a polymorphism rate of 82.7%. Seventeen cultivars had specific genotypes determined using 24 primer pairs and 120 upland cotton cultivars could be identified by only 12 primer combinations. Cluster analysis indicated that genetic similarity coefficient for the 120 upland cotton cultivars ranged from 0.50 to 0.96, with an average of 0.73, indicating that upland cotton resources possess high genetic similarity and have an accordingly narrow genetic basis. [Conclusion] The primer combination method is one of the most effective methods for constructing DNA fingerprinting. The 120 upland cotton varieties were divided into three types with the genetic similarity coefficient matrix; these groups were strongly consistent with their pedigrees.

Key words: Xinjiang; upland cotton; SSR marker; DNA fingerprinting; genetic diversity

中图分类号: 
  • S562.03