DNA Fingerprint Construction and Genetic Diversity Analysis Based on SSR Markers for Upland Cotton in Xinjiang
Wang Xinyi1, Li Xueyuan2, Gong Zhaolong2, Wang Junduo2, Fan Liping1, Zheng Juyun2, Liang Yajun2, Guo Jiangping2, Mamat Moming2, Ai Xiantao2*
1. Key Laboratory of Agriculture Biological Technology / College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China; 2. Economic CropResearch Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
Abstract：[Objective] The aim of this study was to construct a DNA fingerprinting database of 120 upland cotton cultivars from Xinjiang and to analyze their genetic diversity based on SSR markers. [Methods] Seventy-eight evenly distributed SSR primer pairs with high polymorphism and good repeatability were successfully screened out from 586 candidates to construct the fingerprinting database. [Result] A total of 392 alleles from 120 varieties were screened using 78 pairs of core primers, 324 of which were polymorphic loci with a polymorphism rate of 82.7%. Seventeen cultivars had specific genotypes determined using 24 primer pairs and 120 upland cotton cultivars could be identified by only 12 primer combinations. Cluster analysis indicated that genetic similarity coefficient for the 120 upland cotton cultivars ranged from 0.50 to 0.96, with an average of 0.73, indicating that upland cotton resources possess high genetic similarity and have an accordingly narrow genetic basis. [Conclusion] The primer combination method is one of the most effective methods for constructing DNA fingerprinting. The 120 upland cotton varieties were divided into three types with the genetic similarity coefficient matrix; these groups were strongly consistent with their pedigrees.
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