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棉花学报 ›› 2019, Vol. 31 ›› Issue (1): 64-71.doi: 10.11963/1002-7807.wxjlrx.20181228

• 研究与进展 • 上一篇    下一篇

棉花施用脱叶剂对相邻未着药叶片生理活性的影响

王晓婧1(),李思嘉1,刘瑞显1,2,*(),张国伟1,杨长琴1,2,倪万潮1,2   

  1. 1.江苏省农业科学院经济作物研究所/农业农村部长江下游棉花与油菜重点实验室,南京 210014
    2.江苏省现代作物生产协同创新中心,南京 210014
  • 收稿日期:2018-07-20 出版日期:2019-01-15 发布日期:2019-01-15
  • 通讯作者: *刘瑞显 E-mail:wangxiaojing0213@126.com;liuruixian2008 @163.com
  • 作者简介:王晓婧(1989―), 女, 硕士, wangxiaojing0213@126.com
  • 基金资助:
    国家重点研发计划(2017YFD0201900);农业农村部长江下游棉花与油菜重点实验室开放课题(2017-CR01)

Effect of Defoliants Application on Physiological Characters of Cotton Leaf without Defoliants

Wang Xiaojing1(),Li Sijia1,Liu Ruixian1,2,*(),Zhang Guowei1,Yang Changqin1,2,Ni Wanchao1,2   

  1. 1. Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rape in Lower Reaches of Yangtze River of Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu 210014, China
    2. Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu 210014, China
  • Received:2018-07-20 Online:2019-01-15 Published:2019-01-15
  • Contact: *Liu Ruixian E-mail:wangxiaojing0213@126.com;liuruixian2008 @163.com

摘要:

【目的】研究脱叶剂噻苯隆(Thidiazuron, TDZ)处理后,未着药叶片对脱叶剂的响应机理。【方法】以中棉所 50 为材料,分析 TDZ 涂抹叶片不同时间(1 d、3 d、6 d、10 d)后其相邻未着药棉花叶片的光合与叶绿素荧光特性、内源保护酶活性及内源激素含量等的变化。【结果】棉花叶片涂抹 TDZ 6 d后着药叶片脱叶率达到100%,而其相邻未着药叶片在试验期内(TDZ 处理后10 d)无脱落。在 TDZ 施用的前期,未着药棉花叶片净光合速率(Pn)迅速降低,之后逐渐升高恢复至清水对照(CK)水平。TDZ 施用亦降低了未着药棉花叶片光系统II的最大光化学效率(Fv/Fm)、PSII 量子产量(ΦPSII)、电子传递速率(Ret)和光化学猝灭系数(qP),提高了非光化学猝灭系数(qNP);但在处理后10 d,各叶绿素荧光参数逐渐恢复到 CK 水平。TDZ 施用显著提高了相邻未着药棉花叶片的超氧化物歧化酶活性,对过氧化物酶活性影响较小,而降低了丙二醛含量。TDZ 施用后的 3 d、6 d,未着药叶片的脱落酸(Abscisic acid, ABA)含量显著低于 CK,而在处理后 10 d,ABA 含量与 CK 无差异;TDZ 处理对未着药棉花叶片的生长素含量影响较小。【结论】棉花施用噻苯隆显著影响相邻未着药叶片的光合、叶绿素荧光特性、内源保护酶活性及内源激素含量,但药后10 d时未着药叶片的生理活性逐渐恢复到正常水平,这可能是其未脱落的部分原因。

关键词: 棉花; 噻苯隆; 光合特性; 叶绿素荧光参数; 内源保护酶活性; 内源激素含量

Abstract:

[Objective]The aim of this study is to elucidate the physiological mechanism of cotton leaf without defoliant response to thidiazuron (TDZ). [Method] A pot experiment was conducted to study the effect of defoliants on the photosynthesis, chlorophyll fluorescence, antioxidant enzyme activity, and endogenous hormone contents of cotton leaf without defoliant at 1 d, 3 d, 6 d and 10 d after the application of TDZ. Cultivar CCRI-50 was used for this experiment. [Result] TDZ rapidly induced cotton leaf abscission, however, no abscission was detected to the cotton leaf without defoliants. The net photosynthetic rate (Pn) of cotton leaf without defoliants was reduced by 1 d, 3 d and 6 d after TDZ applied, but rise to the control treated with water level by 10 d. TDZ also caused a significant decline in the maximum photochemical efficiency of photosystem II (Fv/Fm), the quantum yield of electron transport (ΦPSII), the electron transport rate (Ret) and the photochemical quenching coefficient (qP), but an increase in the non-photochemical quenching coefficient (qNP). However, with the same changes as Pn, the chlorophyll fluorescence characters got to the control by 10 d. TDZ application significantly increased the superoxide dismutase activity, did not affect the peroxidase activity, decease the malondialdehyde content. Abscisic acid contents in the cotton leaf without defoliant were lower than control by 3 d and 6 d after the applied of TDZ, but have the same level with control at 10 d, and the auxin had a little changes compared with control during the whole period of experimental time. [Conclusion] TDZ application significantly affect the photosynthesis, chlorophyll fluorescence, antioxidant enzyme activity, and endogenous hormone contents of cotton leaf without defoliant. When the leaf with defoliant drop off, the physiological characters of cotton leaf without of defoliants recovered to control level, and no abscission were detected.

Key words: cotton; Thidiazuron; photosynthesis; chlorophyll fluorescence; endogenous protective enzyme activity; endogenous hormone content