棉花轮纹斑病接种方法的优化

doi:10.11963/1002-7807.wjqfj.20180917

棉花学报 ›› 2018, Vol. 30 ›› Issue (6): 486-491.doi: 10.11963/1002-7807.wjqfj.20180917

棉花轮纹斑病接种方法的优化

王婧^1,2，翟伟卜¹，孟菁¹，高环¹，史金艳¹，张文蔚¹，韩榕²，齐放军^1*

1.中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室，北京 100193；2.山西师范大学生命科学学院，山西临汾 041004

收稿日期:2018-04-10 出版日期:2018-11-15 发布日期:2018-11-15
通讯作者: 齐放军，研究员，fjqi@ippcaas.cn
作者简介:王婧(1992―)，女，硕士研究生，534558067@qq.com
基金资助:
国家自然科学基金（31371898）

Optimization of an Inoculation Method for Cotton Alternaria Leaf Spot

Wang Jing^1,2, Zhai Weibo¹, Meng Jing¹, Gao Huan1, Shi Jinyan¹, Zhang Wenwei¹, Han Rong², Qi Fangjun^1*

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Life Science, Shanxi Normal University, Linfen, Shanxi 041004, China

Received:2018-04-10 Online:2018-11-15 Published:2018-11-15

摘要/Abstract

摘要： 【目的】棉花轮纹斑病是严重危害棉花生产的叶部病害。本文旨在优化棉花轮纹斑病菌链格孢（Alternaria alternata）的接种方法。【方法】利用链格孢孢子悬浮液，比较研究了涂菌+套袋保湿接种、涂菌+喷雾保湿接种和喷菌+喷雾保湿接种的效果。【结果】采用涂菌+喷雾保湿接种，接种的棉花叶片易于发病，且能有效区分抗病与感病品种，但涂菌法伤叶不均匀，叶片发病随机性大，存在一定的不均匀性；喷菌+喷雾保湿接种，接种的棉花叶片发病周期相对较长，病程较为缓慢，但和另外2种接种方法相比，发病的一致性好。【结论】喷菌+喷雾保湿接种是1种更适合棉花链格孢抗性研究的接种方法。

关键词: 棉花; 轮纹斑病; 链格孢; 接种方法; 方法优

Abstract: [Objective] Cotton Alternaria leaf spot disease causes serious damage during cotton production. The aim of this study was the optimization of an inoculation method for cotton Alternaria leaf spot. [Method] An Alternaria alternata spore suspension was used as the inoculum. Three inoculation methods were tested and compared: smearing with spores plus retaining moisture using a sealed plastic bag; smearing with spores plus retaining moisture by intermittently spraying with water; and spraying spores plus retaining moisture by intermittently spraying with water. [Result] Inoculated cotton leaves were most readily infected by smearing with spores plus intermittently spraying with water. This inoculation method was effective for distinguishing resistant cotton cultivars from susceptible ones, but often caused nonuniform disease lesions on cotton leaves; thus, the incidence of diseased leaves was random and somewhat nonuniform. Spraying spores plus retaining moisture by intermittently spraying with water led to a relatively longer disease pathogenesis and slower disease progression; however, the incidence of diseased leaves was relatively consistent and uniform, thus providing repeatable inoculation experimental conditions compared with the other two inoculation methods. [Conclusion] Spraying spores plus retaining moisture by intermittently spraying with water is a suitable inoculation method for studying cotton resistance to Alternaria leaf spot.

Key words: cotton; Alternaria leaf spot; Alternaria alternata; inoculation method; method optimization

中图分类号:

S435.621

王婧, 翟伟卜, 孟菁, 高环, 史金艳, 张文蔚, 韩榕, 齐放军. 棉花轮纹斑病接种方法的优化[J]. 棉花学报, 2018, 30(6): 486-491.

Wang Jing, Zhai Weibo, Meng Jing, Gao Huan, Shi Jinyan, Zhang Wenwei, Han Rong, Qi Fangjun. Optimization of an Inoculation Method for Cotton Alternaria Leaf Spot[J]. Cotton Science, 2018, 30(6): 486-491.

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棉花轮纹斑病接种方法的优化

Optimization of an Inoculation Method for Cotton Alternaria Leaf Spot

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