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棉花学报 ›› 2018, Vol. 30 ›› Issue (2): 155-163.doi: 10.11963/1002-7807.sdytfy.20180228

• 研究与进展 • 上一篇    下一篇

花铃期土壤干旱对棉花叶片抗氧化及光合作用的影响

邵德意#,罗海华#,陈功,刘云涛,高欣,汤飞宇*   

  1. 江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室,南昌 330045
  • 收稿日期:2017-06-22 出版日期:2018-03-15 发布日期:2018-03-15
  • 通讯作者: fytangcau@163.com
  • 作者简介:邵德意(1992―),男,硕士,shaodeyi920519@163.com;罗海华,lhhjxcotton@163.com; #同等贡献。
  • 基金资助:
    国家自然科学基金(31260302)

Antioxidant and Photosynthetic Responses of Cotton to Soil Drought Stress during Flowering and Fruiting Stage

Shao Deyi #, Luo Haihua #, Chen Gong, Liu Yuntao, Gao Xin, Tang Feiyu*   

  1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, College of Agronomy, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2017-06-22 Online:2018-03-15 Published:2018-03-15

摘要: 【目的】探讨棉铃对位果枝叶介导不同的生化调节物质(抗氧化保护酶、渗透调节物质等)及光合作用参数对花铃期土壤干旱的响应方式。【方法】以陆地棉品系A001和A705为供试材料,采用盆栽法,于2016年种植在温室大棚内。在不同土壤含水量处理(正常灌溉、干旱)下,研究棉铃发育期间对位果枝叶抗氧化酶(过氧化物酶,peroxidase,POD)、渗透调节物质(脯氨酸)、膜脂过氧化产物(丙二醛,malondialdehyde,MDA)、可溶性蛋白及主茎叶光合参数(净光合速率,net photosynthetic rate,Pn;蒸腾速率,transpiration rate,E;气孔导度,stomatal conductance,Gs)的动态变化及基因型差异。【结果】土壤干旱导致A001棉铃对位果枝叶可溶性蛋白含量下降、POD酶活力和MDA含量上升,但对A705的影响在不同铃龄不一致且差异不显著,对脯氨酸的影响因不同基因型和不同铃龄而异。土壤干旱显著降低了A001和A705主茎叶的Pn、E和Gs。【结论】POD、可溶性蛋白和MDA参与了A001和A705棉铃对位果枝叶对土壤干旱胁迫的响应。研究结果可为棉花抗(耐)旱品种选育提供参考。

关键词: 陆地棉; 棉铃对位果枝叶; 干旱胁迫; 抗氧化物; 光合作用

Abstract: [Objective] The purpose of this study was to examine potential drought tolerance mechanisms in cotton (Gossypium  hirsutum L.). Biochemical (antioxidant, protein and compatible osmolyte) and physiological (photosynthesis) responses to drought stress during the flowering and fruiting stages were examined. [Method] Using two cotton genotypes (A001 and A705) with different drought tolerance, a pot study was conducted in 2016 with treatments consisting of control (well-watered) and water stress. Water stress treatment was designed as withholding water from the pots until stomatal closure followed by limited water supply for 25 days where water-stressed plants received 40% of the optimum quantity of water. Measurements were made on soluble protein, proline and malonaldehyde (MDA) concentrations, peroxidase (POD) activity and photosynthetic characteristics (net photosynthetic rate Pn, transpiration rate E, stomatal conductance Gs) during the period of water stress. [Result] The soluble protein concentration was decreased and POD activity and MDA level were increased in the leaf subtending cotton boll of A001 under water deficit when compared with the control, but no response was observed in A705. Proline level responded to water deficit inconsistently between the two genotypes and across all sampling dates. Water stress significantly decreased Pn, E and Gs in the main stem leaves for both A001 and A705. [Conclusion] The results indicated that POD, soluble protein and MDA are involved in A001 and A705 responses to water deficit, and A705 is more tolerant to soil drought than A001.

Key words: Gossypium hirsutum L; leaf subtending cotton boll; drought stress; antioxidant; photosynthesis

中图分类号: 
  • S562.01