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棉花学报 ›› 2018, Vol. 30 ›› Issue (1): 38-44.doi: 10.11963/1002-7807.lycdh.20180102

• 研究与进展 • 上一篇    下一篇

昼夜变温下高温对Bt棉铃壳杀虫蛋白及氮代谢生理的影响

李远,王桂霞,胡大鹏,Abidallah Eltayib,张丽雅,张祥,陈源,陈德华*   

  1. 扬州大学江苏省作物遗传生理重点实验室,江苏 扬州 225009
  • 收稿日期:2016-09-29 出版日期:2018-01-15 发布日期:2018-01-15
  • 通讯作者: cdh@yzu.edu.cn
  • 基金资助:
    国家自然科学基金(31471435,31301263,31671613);江苏省三新工程项目(SXGC(2014)317,(2016)320);江苏省高等教育优势学科发展计划(PAPD);中国农业科学院棉花研究所植棉技术标准化研究与示范团队项目(2016PCTS-1)

Effects of High Alternating Day and Night Temperature Conditions on the Boll Wall's Bt Protein Content and Nitrogen Metabolic Physiology in Bt Cotton

Li Yuan, Wang Guixia, Hu Dapeng, Abidallah Eltayib, Zhang Liya, Zhang Xiang, Chen Yuan, Chen Dehua*   

  1. Key Laboratory of Genetics and Physiology in Yangzhou University, Yangzhou, Jiangsu 225009, China
  • Received:2016-09-29 Online:2018-01-15 Published:2018-01-15

摘要: 【目的】确定在昼夜变温条件下,高温逆境对转基因棉花铃壳中杀虫蛋白含量的影响及相关生理特征。[方法]以大面积推广应用的Bt棉常规品种泗抗1号和杂交种泗抗3号为材料,于2011―2012年在扬州大学遗传生理重点实验室,用盆栽棉花在人工气候室设计昼夜变温(39/27 ℃)条件下高温持续4~10 d胁迫,研究铃壳中Bt蛋白含量、氮代谢相关物质和关键酶活性的变化特征。【结果】铃壳中Bt杀虫蛋白含量随胁迫时间的延长而降低。与对照(32/25 ℃)相比,胁迫7 d后铃壳中Bt杀虫蛋白开始急剧下降。2011年度常规种泗抗1号和杂交种泗抗3号分别下降了18.44%和14.82%;2012年分别下降了19.07%和15.26%。铃壳中Bt杀虫蛋白下降同时,其游离性氨基酸含量和蛋白酶活性明显上升,而可溶性蛋白含量和GPT活性则显著下降。【结论】昼夜变温下高温引起铃壳中蛋白质合成减弱,分解加剧,导致蛋白质含量下降(包括Bt蛋白含量下降),从而引起抗虫性降低。

关键词: Bt棉; 高温胁迫; Bt杀虫蛋白; 氮代谢; 铃壳

Abstract:  [Object] In this study, the objective was to study changes in the boll wall's Bt protein concentration and related physiological characteristics under high alternating day and night temperature conditions in Bt cotton. [Method] The experiments were conducted at the Key Laboratory of Genetics and Physiology, Yangzhou University during the 2011-2012 cotton growing seasons. The conventional Bt cotton cultivar Sikang-1 and hybrid cultivar Sikang-3 were used as the test materials. The boll wall's Bt protein content and nitrogen metabolic physiology were investigated under high temperatures (39/27 ℃, day/night) for 4-10 d at the peak boll stage. [Result] The Bt protein content in the boll wall decreased as the stress time increased. Compared with the control (day/night, 32/25 ℃), the Bt protein content in the boll wall decreased significantly at 7 d under the high temperature stress. The values for cultivars Sikang-1 and Sikang-3 decreased by 18.44% and 14.82%, respectively, in 2011, and by 19.07% and 15.26%, respectively, in 2012. As the Bt toxin content decreased, the boll wall's free amino acid content and protease activity increased, while the soluble protein content and GPT (Glutamic-pyruvic transaminase) activity decreased significantly. [Conclusion] The reduction in Bt protein synthesis and increase in the insecticidal protein degradation at the boll wall under high temperatures caused a reduction in the protein content, including the Bt protein content, which resulted in a reduction in insect resistance.

Key words: Bt cotton; high temperature stress; Bt protein; nitrogen metabolism; boll wall

中图分类号: 
  • S562.01