棉花学报 ›› 2019, Vol. 31 ›› Issue (1): 40-52.doi: 10.11963/1002-7807.lycbl.20181220
李岩1(),张希鹤1,郁凯1,霍钰阳1,王友华1,2,陈兵林1,2,*(
)
收稿日期:
2018-06-06
发布日期:
2019-01-15
通讯作者:
陈兵林
E-mail:2015101007@njau.edu.cn;blchen@njau.edu.cn
作者简介:
李岩(1990—),男,硕士研究生, 基金资助:
Li Yan1(),Zhang Xihe1,Yu Kai1,Huo Yuyang1,Wang Youhua1,2,Chen Binglin1,2,*(
)
Received:
2018-06-06
Published:
2019-01-15
Contact:
Chen Binglin
E-mail:2015101007@njau.edu.cn;blchen@njau.edu.cn
摘要:
【目的】研究黄萎病菌胁迫下钾素对棉花黄萎病抗性生理机制的影响,对棉花黄萎病综合防治技术的提出具有理论指导意义。【方法】在人工黄萎病病圃中,利用3个对黄萎病有不同抗性的品种冀棉11号(JM-11,感病品种)、中植棉2号(ZZM-2,抗病对照)、农大601(ND-601,抗病品种),研究5种施钾量(0、75、150、225、300 kg·hm-2)对棉花黄萎病发生、棉株抗病性相关物质含量及酶活性的影响。【结果】(1)感病品种JM-11抗黄萎病相关物质(木质素、总酚)含量和苯丙氨酸解氨酶、过氧化物酶活性受病情的影响较抗病品种ZZM-2和ND-601大;根和叶木质素、总酚含量多少及棉叶苯丙氨酸解氨酶活性高低影响感病品种JM-11抗黄萎病性,根和叶中总酚是影响抗病品种黄萎病发生程度的关键物质。(2)JM-11根对钾含量的敏感性强于ND-601,弱于ZZM-2,对叶中钾含量的敏感性强于ZZM-2、ND-601。(3)JM-11、ZZM-2、ND-601黄萎病最大缓解效果分别为32.5%、26.4%、16.5%,对应施钾量分别为290.5、253.5、229.5 kg·hm-2。【结论】抗黄萎病性弱的品种通过适量施钾可增强其对黄萎病抗性,减轻黄萎病的发生。
李岩,张希鹤,郁凯,霍钰阳,王友华,陈兵林. 不同棉花品种及施钾量对黄萎病抗性生理机制的影响[J]. 棉花学报, 2019, 31(1): 40-52.
Li Yan,Zhang Xihe,Yu Kai,Huo Yuyang,Wang Youhua,Chen Binglin. Physiological Mechanism of Different Varieties and Potassium Application Amounts on Cotton Resistance to Verticillium Wilt[J]. Cotton Science, 2019, 31(1): 40-52.
表2
不同品种及施钾量缓解棉花黄萎病情效果"
年份 Years | 品种 Cultivars | 拟合方程 Fitting equation | R2 | XYmax / (kg·hm-2) | Ymax / % | Ymean / % |
2016 | JM-11 | Y=3.326+0.164X-1.855×10-4X2 | 0.907** | 300 | 35.8 | 26.1 |
ZZM-2 | Y=-9.637+0.328X-6.567×10-4X2 | 0.880** | 250 | 31.3 | 24.1 | |
ND-601 | Y=-7.407+0.263X-5.659×10-4X2 | 0.891** | 232 | 23.2 | 18.0 | |
2017 | JM-11 | Y=3.925+0.180X-3.205×10-4X2 | 0.720* | 281 | 29.2 | 24.3 |
ZZM-2 | Y=9.079+0.096X-1.868×10-4X2 | 0.744* | 257 | 21.4 | 19.2 | |
ND-601 | Y=-4.006+0.050X-1.100×10-4X2 | 0.743* | 227 | 9.7 | 8.7 |
表3
铃期棉花黄萎病株根和叶中钾含量的变化"
年份 Years | 钾处理 Potassium treatments | JM-11 | ZZM-2 | ND-601 | |||||
---|---|---|---|---|---|---|---|---|---|
根 Roots | 叶 Leaves | 根 Roots | 叶 Leaves | 根 Roots | 叶 Leaves | ||||
2016 | K75 | 3.9 | 7.8 | 1.0 | 18.7 | 8.1 | 2.0 | ||
K150 | 3.5 | 11.6 | 1.2 | 20.3 | 7.4 | 4.8 | |||
K225 | 5.2 | 10.9 | 1.4 | 26.9 | 13.2 | 7.9 | |||
K300 | 6.0 | 13.1 | 1.2 | 25.2 | 10.1 | 6.6 | |||
平均Mean | 4.7 | 10.9 | 1.2 | 22.8 | 9.7 | 5.3 | |||
2017 | K75 | 4.4 | 8.0 | 5.1 | 13.6 | 9.7 | 2.9 | ||
K150 | 5.3 | 9.7 | 5.2 | 19.5 | 11.7 | 1.2 | |||
K225 | 6.5 | 10.9 | 5.7 | 19.9 | 11.6 | 4.3 | |||
K300 | 6.8 | 12.4 | 5.2 | 18.6 | 11.4 | 4.2 | |||
平均Mean | 5.8 | 10.3 | 5.3 | 17.9 | 11.1 | 3.2 |
表4
铃期不同品种棉花黄萎病情与发病棉株钾浓度、抗病相关物质含量及酶活性的相关系数"
年份 Years | 品种 Varieties | 钾含量 K contents | 木质素含量 Lignin contents | 总酚含量 Total phenols contents | 叶苯丙氨酸解氨酶活性Leaf PAL activities | 叶过氧化物酶活性Leaf POD activities | ||||||
根 Roots | 叶 Leaves | 根 Roots | 叶 Leaves | 根 Roots | 叶 Leaves | |||||||
2016 | JM-11 | -0.950* | -0.921* | -0.931* | -0.939* | -0.893* | -0.951* | -0.957* | -0.869 | |||
ZZM-2 | -0.901* | -0.978** | -0.874 | -0.803 | -0.900* | -0.923* | -0.824 | -0.835 | ||||
ND-601 | -0.947* | -0.858 | 0.828 | 0.864 | -0.968** | -0.912* | 0.864 | -0.607 | ||||
2017 | JM-11 | -0.984** | -0.954* | -0.999** | -0.944** | -0.894* | -0.973** | -0.933* | -0.867 | |||
ZZM-2 | -0.895* | -0.980** | -0.831 | -0.937* | -0.961** | -0.963** | -0.712 | -0.887* | ||||
ND-601 | -0.984** | -0.582 | -0.921* | -0.848 | -0.884* | -0.949* | -0.622 | -0.474 |
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