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棉花学报 ›› 2019, Vol. 31 ›› Issue (2): 114-120.doi: 10.11963/1002-7807.lsmsgl.20190325

• 研究与进展 • 上一篇    下一篇

棉花光籽基因n2的精细定位

李思敏1,2(),左东云2,程海亮2,张友平2,王巧连2,刘珂2,冯晓旭2,耿洪伟1,宋国立2,*()   

  1. 1.新疆农业大学,乌鲁木齐 830052
    2.中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2018-04-19 出版日期:2019-03-15 发布日期:2019-03-15
  • 通讯作者: 宋国立 E-mail:lisimin1217@126.com;sglzms@163.com
  • 作者简介:李思敏(1990-),女,硕士研究生, lisimin1217@126.com
  • 基金资助:
    国家重点研发计划(2018YFD0100402)

Fine Mapping of the Fuzzless Gene n2 in Cotton

Li Simin1,2(),Zuo Dongyun2,Cheng Hailiang2,Zhang Youping2,Wang Qiaolian2,Liu Ke2,Feng Xiaoxu2,Geng Hongwei1,Song Guoli2,*()   

  1. 1. Xinjiang Agricultural University, Urumqi 830052, China
    2. Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2018-04-19 Online:2019-03-15 Published:2019-03-15
  • Contact: Song Guoli E-mail:lisimin1217@126.com;sglzms@163.com

摘要:

【目的】棉纤维是由胚珠外珠被表皮单细胞经突起并极性伸长而成的,棉花种子上的短绒是由胚珠外珠被表皮单细胞突起而产生的。因此,本研究对棉纤维光籽基因n2进行定位,旨在为其克隆和功能研究奠定基础。【方法】以海岛棉新海21和陆地棉光籽突变体n2为亲本构建F2群体,结合棉花基因组数据,开发简单序列重复标记,定位该基因,获得预测的基因序列,通过实时荧光定量聚合酶链式反应分析候选基因的表达量差异。【结果】光籽基因n2被定位在染色体A12上,位于标记P61与Z10之间的181 kbp内。该区间共有7个候选基因。实时荧光定量聚合酶链式反应分析显示,候选基因72098在2个亲本中的表达量有显著差异。【结论】本试验精细定位了光籽基因n2,初步分析了候选基因,为其图位克隆和功能验证奠定了基础。

关键词: 陆地棉; 纤维; 光籽突变体; 精细定位; 表达分析

Abstract:

[Objective] Cotton fibers are single cells derived from the ovule epidermis, cotton fuzz of seeds are also formed by the ovule epidermis. Therefore, we conduct this experiment to locate the fuzzless gene n2 so as to lay a foundation for its clone and function study. [Method] Two F2 populations derived from a cross between the Xinhai 21 and the fuzzless mutant of upland cotton (Gossypium hirsutum L.) n2 was constructed. Combined with cotton genomic data and developed simple sequence repeat markers to locate the gene. The predicted gene sequence was acquired and the differences in its expression in two parents were analyzed by using the quantitative real time polymerase chain reaction method. [Result] The fuzzless gene was located in a 181 kbp region on chromosome A12 within markers P61 and Z10. There are seven candidate genes in the region. Quantitative real time polymerase chain reaction verification shows significant differences in the expression of 72098 gene in two parents. [Conclusion] In this experiment, the fine mapping and candidate genes analysis of gene n2 were completed, which laid a foundation for n2 gene cloning and functional verification.

Key words: Upland cotton; fiber; fuzzless mutant; fine mapping; expression analysis