欢迎访问《棉花学报》! 今天是

棉花学报 ›› 2019, Vol. 31 ›› Issue (3): 254-262.doi: 10.11963/1002-7807.lnnwhm.20190515

• 研究与进展 • 上一篇    

基于RIL群体鉴定棉花抗黄萎病相关QTLs

鲁宁宁(),赵云雷,王红梅*(),陈伟,赵佩,龚海燕,崔艳利,桑晓慧,张凯   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室郑州大学研究基地,河南 安阳 455000
  • 收稿日期:2017-10-26 发布日期:2019-05-30
  • 通讯作者: 王红梅 E-mail:luningkf@126.com;aywhm@163.com
  • 作者简介:鲁宁宁(1993―),女,硕士研究生, luningkf@126.com
  • 基金资助:
    中国农业科学院科技创新工程(CAAS-ASTIP-2017-ICR)

Identification of QTLs Related to Verticillium Wilt Resistance Based on RIL Population

Lu Ningning(),Zhao Yunlei,Wang Hongmei*(),Chen Wei,Zhao Pei,Gong Haiyan,Cui Yanli,Sang Xiaohui,Zhang Kai   

  1. Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base, Zhengzhou University, State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2017-10-26 Published:2019-05-30
  • Contact: Wang Hongmei E-mail:luningkf@126.com;aywhm@163.com

摘要:

【目的】鉴定出能够稳定表达的棉花抗黄萎病相关数量性状位点(Quantitative trait loci,QTLs)。【方法】以抗落叶型黄萎病棉花品种常抗棉和感黄萎病品种TM-1为亲本配制的111个重组自交系家系为作图群体,筛选出多态性简单序列重复(Simple sequence repeat,SSR)标记,并用于构建遗传图谱。用完备复合区间作图法对该群体在安阳大田、新疆重病地及病圃等多个环境下的黄萎病病情指数进行QTLs检测。【结果】构建了1张含有12个连锁群、40个标记、总长212.5 cM(厘摩)的遗传图谱。获得了6个与抗黄萎病基因相关的QTLs,对数优势比(Logarithm of the odd score,LOD)分布在2.51~5.55,贡献率最大为20.34%,最小为6.93%。其中,qVR-D05-1能够在安阳大田2015年7月15日和新疆南疆重病地2016年7月9日2个环境中检测到,贡献率分别为12.96%和20.34%。【结论】本研究得到的qVR-D05-1能够为定位出稳定的棉花抗黄萎病相关QTLs提供参考。

关键词: 棉花; SSR; 黄萎病; 遗传图谱; 数量性状位点

Abstract:

[Objective] The objective of this study was to identify the stable quantitative trait loci (QTLs) related to Verticillium wilt resistance in cotton. [Method] In this study, a population of 111 recombinant inbred lines (RILs) strains was developed by crossing a highly resistant parental line "Changkangmian" to Verticillium wilt and the susceptible parent TM-1. The complete composite interval mapping method was adopted to detect QTLs by Verticillium wilt disease index in multiple environmental conditions and periods in Anyang and Verticillium wilt affected areas of Xinjiang. Simple sequence repeat (SSR) markers of polymorphism were screened for genetic mapping. [Result] The genetic map was constructed by 40 simple sequence repeat (SSR) markers, consisted of 12 linkage groups with total length of 212.5 centimorgan (cM). A total of six QTLs related to the resistance to Verticillium wilt were obtained. The likelihood of odd (LOD) values ranged from 2.51 to 5.55. The maximum phenotypic variation explained (PVE) 20.34%, and the minimum PVE 6.93% were recorded. Among detected QTLs, qVR-D05-1 was detected in both Verticillium wilt affected fields in Anyang and Xinjiang in July 2015 and July 2016 with PVE of 12.96% and 20.34%, respectively. [Conclusion] This study can provide a potential reference for mapping stable QTLs related to resistance to Verticillium wilt.

Key words: cotton; SSR; Verticillium wilt; genetic linkage map; quantitative trait loci