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棉花学报 ›› 2019, Vol. 31 ›› Issue (2): 129-137.doi: 10.11963/1002-7807.lmzzg.20190314

• 研究与进展 • 上一篇    下一篇

湖南省棉花黄萎病菌致病力分化及致病型分布研究

李敏1,2#(),李彩红2#(),赵瑞元2,刘冰蕾2,张志刚2,*()   

  1. 1.湖南农业大学,长沙 410128
    2.湖南省棉花科学研究所,湖南 常德 415101
  • 收稿日期:2019-01-04 出版日期:2019-03-15 发布日期:2019-03-15
  • 通讯作者: 张志刚 E-mail:285136548@qq.com;lch19860616@163.com;zhangzhig@126.com
  • 作者简介:李敏(1993-),男,硕士研究生, 285136548@qq.com。|李彩红(1986-),女,主要从事植物病害研究, lch19860616@163.com
  • 基金资助:
    国家重点研发计划(2017YFD0201900);湖南农业科技创新资金(2017XC03)

Pathogenicity Differentiation and Pathotype Distribution of Verticillium dahliae from Cotton in Hunan Province

Li Min1,2#(),Li Caihong2#(),Zhao Ruiyuan2,Liu Binglei2,Zhang Zhigang2,*()   

  1. 1. Hunan Agricultural University, Changsha 410128, China
    2. Hunnan Cotton Science Institute, Changde, Hunan 415101, China
  • Received:2019-01-04 Online:2019-03-15 Published:2019-03-15
  • Contact: Zhang Zhigang E-mail:285136548@qq.com;lch19860616@163.com;zhangzhig@126.com

摘要:

【目的】研究湖南主产棉区黄萎病菌致病性变异情况,为湖南棉花抗病品种选育推广及棉花黄萎病综合防控提供理论依据。【方法】对湖南省各主产棉县(区)分离的77个黄萎病菌单孢进行培养特性观察,测定其中31个菌株生长速率、产孢量和致病力,并以SAS 9.1.3统计分析软件对供试菌株致病力聚类。以特异性引物(D-1/D-2 和ND-1/ND-2)经聚合酶链式反应检测24个菌株的致病类型。【结果】根据微菌核产量及在马铃薯葡萄糖琼脂培养基上培养的菌落特性,菌株可划分为菌核型、菌丝型和中间型3种培养类型,分别占总菌株数的14.28%、42.86%和42.86%。供试菌株生长速率为1.22~2.54 mm·d-1,产孢量为5.3×106~40.6×106 mL-1,各菌株之间存在明显差异。根据平均病情指数聚类结果将供试菌株划分为致病力强的Ⅰ型、致病力弱的Ⅱ型和致病力中等的Ⅲ型,分别占3.2%、12.9%和83.9%。【结论】湖南省棉花黄萎病菌以菌丝型和中间型为主要培养类型,且致病力存在明显分化;致病型检测结果表明目前湖南主产棉区黄萎病菌以落叶型为主。

关键词: 湖南; 棉花黄萎病菌; 致病力; 遗传分化

Abstract:

[Objective] The study of pathogenicity differentiation of Verticillium dahliae in the cotton growing areas in Hunan province could provide theories for breeding and promoting resistant varieties as well as integrated control of Verticillium wilt of cotton. [Method] Seventy seven strains of V. dahliae from major cotton growing areas in Hunan province were evaluated their cultural characteristics. The growth rate, sporulation quantity and pathogenicity of 31 strains among them were tested and SAS 9.1.3 statistical analysis software was used to cluster the pathogenicity of the tested strains. The special primer (D-1/D-2 and ND-1/ND-2) for polymerase chain reaction assay was used to test pathotype of 24 strains. [Result] According to their colony morphology on potato dextrose agar media and culture characteristics, the strains were classified into the sclerotium type, the hyphal type and the intermediate type accounted for 14.28%, 42.86% and 42.86%, respectively. The growth rate and sporulation quantity of the strains were 1.22-2.54 mm·d-1 and 5.3×106-40.6×106 spore·mL-1, which showed significant difference. In addition, based on the clustering results of average disease index,the strains were clustered into three groups which showed high (Type Ⅰ), low (Type Ⅱ) and moderate (Type Ⅲ) virulence to cotton with the proportion of 3.2%, 12.9% and 83.9%, respectively. [Conclusion] It is conclude that the main culture types of V. dahliae in Hunan province are the hyphal type and the intermediate type, and the strains showed pathogenicity differentiation. The result showed that defoliating pathotype of V. dahliae was dominant in the cotton growing areas of Hunan province.

Key words: Hunan province; Verticillium dahliae; pathogenicity; genetic variation