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棉花学报 ›› 2020, Vol. 32 ›› Issue (5): 381-391.doi: 10.11963/1002-7807.ljlb.20200729

• 研究与进展 •    下一篇

冠菌素对低温胁迫下棉花幼苗AsA-GSH循环的调控效应研究

李进1,翟梦华2,于春欣3,王莉2,张军高1,周小云1,梁晶1,段留生3*,雷斌1*   

  1. 1. 新疆农业科学院核技术生物技术研究所/农业部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐 830091;2. 新疆农业大学农学院,乌鲁木齐 830052;3. 中国农业大学农学院/植物生长调节剂教育部工程研究中心,北京 10019
  • 收稿日期:2020-03-27 出版日期:2020-09-15 发布日期:2020-09-29
  • 通讯作者: 段留生, duanlsh@cau.edu.cn;雷斌,leib668@xaas.ac.cn
  • 作者简介:李进(1990―), 男,助理研究员,xjnky001@163.com
  • 基金资助:
    国家重点研发计划项目(2017YFD0201300);农业部荒漠绿洲作物生理生态与耕作重点实验室开放课题(25107020-201905)

Effect of Coronatine on AsA-GSH Cycle of Cotton Seedling under Low Temperature Stress

Li Jin1, Zhai Menghua2, Yu Chunxin3, Wang Li2, Zhang Jungao1, Zhou Xiaoyun1, Liang Jing1, Duan Liusheng3*,Lei Bin1*   

  1. 1. Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System in Desert Oasis Region, Ministry of Agriculture, Urumqi 830091, China; 2. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China; 3. College of Agronomy and Biotechnology, China Agricultural University/Engineering Research Center of Plant Growth Regulator, Ministry of Education, Beijing 100193, China
  • Received:2020-03-27 Online:2020-09-15 Published:2020-09-29

摘要: 【目的】研究冠菌素(Coronatine, COR)对低温胁迫下棉花幼苗营养器官抗坏血酸-谷胱甘肽循环系统的影响,探索其抗逆机理。【方法】以新陆早57号为材料,设4个处理,幼苗2叶期叶面喷施清水后进行25 ℃(CK)和4 ℃(LT)处理,幼苗2叶期叶面喷施冠菌素(0.01 μmol·L-1)后进行25 ℃(COR)和4 ℃(LT+COR)处理,1 d后采集各处理根、茎和叶,测定抗氧化物质含量和酶活性。【结果】与CK相比,LT处理引起棉花幼苗根、茎和叶中抗坏血酸过氧化物酶和单脱氢抗坏血酸还原酶活性、氧化型抗坏血酸、还原型谷胱甘肽和谷胱甘肽含量下降,谷胱甘肽过氧化物酶活性、还原型抗坏血酸和抗坏血酸含量升高,脱氢抗坏血酸还原酶和谷胱甘肽还原酶活性无差异,叶中氧化型谷胱甘肽含量减少。与LT相比,(LT+COR)处理使棉花幼苗根、茎和叶中抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性升高,还原型抗坏血酸、氧化型抗坏血酸、还原型谷胱甘肽、抗坏血酸和谷胱甘肽含量增加,氧化型谷胱甘肽含量、还原型抗坏血酸与氧化型抗血酸的含量比、还原型谷胱甘肽与氧化型谷胱甘肽含量比在叶中变化明显。【结论】COR可以调控棉花幼苗抗坏血酸-谷胱甘肽循环系统,缓解低温对棉花幼苗造成的损伤,对叶片缓解作用最强。

关键词: 棉花; 低温胁迫; 冠菌素; 抗坏血酸-谷胱甘肽循环; 生理特性

Abstract: [Objective] In order to explore the stress-resistant mechanism of coronatine, an indoor experiment was conducted to study the effect of coronatine on ascorbate-glutathione circulatory system of the vegetative organs of cotton seedling under low temperature stress. [Method] Xinluzao 57 was used as the experimental material, when the seedlings reached the two-leaf stage, they were treated at 25 ℃ and 4 ℃ after spraying water, marked as CK and LT; the same treatment was conducted after spraying 0.01 μmol·L-1 coronatine, marked as COR and (LT+COR). After 1 d of treatment, the roots, stems and leaves of cotton seedling were collected to determine the antioxidant contents and antioxidant enzyme activities. [Result] Compared with the control treatment (CK), the APX, MDHAR activities and DHA, GSH, glutathione contents decreased, the GPX activity, AsA and ascorbate contents increased, and no significant effect on DHAR and GR activities were observed in roots, stems and leaves of cotton seedling after spraying water at low temperature (LT), while in leaves, the GSSG content decreased, AsA-DHA ratio and GSH-GSSG ratio showed the maximum increase. Compared with the LT treatment, spraying COR at low temperature (LT+COR) showed increased APX, MDHAR, DHAR, GPX, GR activities and AsA, DHA, GSH, ascorbate contents; glutathione content in roots, stems and leaves of cotton seedling, and GSSG content, AsA-DHA ratio and GSH-GSSG ratio obviously changed in leaves. [Conclusion] Spraying COR at low temperature could regulate the AsA-GSH metabolism and alleviate the damage caused by low temperature to cotton seedling. COR had the strongest relieving effect on seedling leaves.

Key words: cotton; low temperature stress; coronatine; AsA-GSH cycle; physiological characteristics