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棉花学报 ›› 2017, Vol. 29 ›› Issue (4): 336-344.doi: 10.11963/1002-7807.kdplfg.20170703

• 研究与进展 • 上一篇    下一篇

亚洲棉EMS诱变条件优化与突变体筛选

孔德培1,2,屈凌波1,张雪妍2,刘记2,王鹏2,李付广2*   

  1. 1.河南工业大学/生物工程学院,郑州 450001;2.中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2017-03-29 出版日期:2017-07-15 发布日期:2017-07-15
  • 通讯作者: aylifug@163.com
  • 作者简介:孔德培(1987―),男,硕士研究生,助理研究员,kongdepei1987@163.com
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项(1610162016011);国家自然科学基金联合基金(U1303282)

Optimization of EMS Mutagenesis Condition and Screening of Mutants in Gossypium arboretum L.

Kong Depei1,2, Qu Lingbo1, Zhang Xueyan2, Liu Ji2, Wang Peng2, Li Fuguang2*   

  1. 1. Henan University of Technology, Zhengzhou 450001, China; 2. State Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China
  • Received:2017-03-29 Online:2017-07-15 Published:2017-07-15

摘要: 【目的】明确适于亚洲棉诱变的甲基磺酸乙酯(EMS)最佳浓度和处理时间,创制优异亚洲棉突变体。【方法】用体积分数0.4%~1.5% EMS溶液浸泡处理亚洲棉石系亚1号种子4~8 h,分析不同时间、不同浓度的EMS溶液处理对石系亚1号种子萌发和生长的影响,并对M1、M2代突变株的形态学特征进行了初步鉴定。【结果】0.6% EMS溶液处理8 h,石系亚1号种子的发芽率、发芽指数分别为51%、18.84,诱变效果最佳。以该参数大规模诱变处理约23 000粒石系亚1号种子,共筛选获得M1代变异材料5559株,突变频率为48.37%;M2代变异材料825份,突变频率为14.17%,其中叶型、株型的突变频率最高,分别为8.0%,5.9%;M3代变异株系57个,变异性状遗传频率为31.30%,M3代新增突变频率为18.26%。【结论】本研究建立了亚洲棉种子EMS诱变体系,构建了亚洲棉石系亚1号M2突变群体,获得了36份可稳定遗传的突变体株系,为棉花功能基因组研究奠定了材料基础。

关键词: 甲基磺酸乙酯; 亚洲棉; 突变体库; 变异性状; 石系亚1号

Abstract: [Objective] This research aimed to clarify the optimization of EMS concentration and treat duration on mutation of Gossypium arboretum L. and create excellent mutants library. [Method] Seeds of Gossypium arboreum line Shixiya1 were immersed in EMS solutions with volume fraction ranging from 0.4% to 1.5% by 4-8 h. Then, the seed germination index and seedling growth were analyzed and the morphological character of M1, M2, and M3 generation were identified. [Result] The treatment with 0.6% EMS for 8 h was appropriate for Shixiya 1 mutagenesis. Under this treatment conditions, the seed germination index and the germination rate were 18.84 and 51%, respectively. To obtain abundant mutants, approximated 23 000 seeds of Shixiya 1were treated with 0.6% EMS for 8 h. 5559 mutants were screened out from M1 generation, 825 and 57 from M2 and M3, respectively. The mutation frequency of Shixiya 1 was 48.37%. The mutation frequency of leaf type variants, plant type variants were the highest in M2, which were 8.0% and 5.9%, respectively.The inheritable frequency of mutant characters from M2 generation to M3 generation was 31.30%, and the mutant frequency of M3 generation was 18.26%. [Conclusion]This study have established the EMS mutagenesis system of Gossypium arboreum line Shixiya 1, and created 36 steadily inherited mutants. The mutant library we constructed will be a very useful genetic resource for functional genomics and genetic improvement in cotton.

Key words: ethyl methanesulfonate; Gossypium arboretum L.; mutant library; variation character; Shixiya 1

中图分类号: 
  • S562.035.2