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棉花学报 ›› 2021, Vol. 33 ›› Issue (2): 124-133.doi: 10.11963/1002-7807.jxywgy.20210226

• 研究与进展 • 上一篇    下一篇

陆地棉株型及生育期相关性状QTL定位

贾晓昀(),王士杰,赵红霞,朱继杰,李妙(),王国印()   

  1. 河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,石家庄 050035
  • 收稿日期:2020-09-27 出版日期:2021-03-15 发布日期:2021-04-07
  • 通讯作者: 李妙,王国印 E-mail:jiaxiaoyun1987@163.com;limiao2003@sina.com;wgy1963@vip.sina.com
  • 作者简介:贾晓昀(1987―),男,博士, jiaxiaoyun1987@163.com
  • 基金资助:
    河北省农林科学院博士科研项目(C19R0301);河北省农林科学院粮油作物研究所青年创新基金(2018LYS01);河北省农业创新工程项目(2019-7-3-5);河北省现代农业产业技术体系棉花产业创新团队建设(HBCT2018040202);河北省重点研发计划项目(19226324D)

QTL analysis for Gossypium hirsutum L. plant architecture and growth period traits

Jia Xiaoyun(),Wang Shijie,Zhao Hongxia,Zhu Jijie,Li Miao(),Wang Guoyin()   

  1. Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Science / Hebei Laboratory of Crop Genetics and Breeding, Shijiazhuang 050035, China
  • Received:2020-09-27 Online:2021-03-15 Published:2021-04-07
  • Contact: Li Miao,Wang Guoyin E-mail:jiaxiaoyun1987@163.com;limiao2003@sina.com;wgy1963@vip.sina.com

摘要:

【目的】 为了深入分析棉花株型及生育期相关性状的分子遗传机制,加速适机采棉花新品种分子标记辅助育种进程。【方法】 通过构建包含413个单株的F2群体,结合高密度SNP(Single nucleotide polymorphism,单核苷酸多态性)遗传图谱,开展株高(Plant height,PH)、第一果枝节位(Node of the first fruiting branch,NFFB)及其高度(Height of NFFB,HNFFB)、第四果枝第一果节长度(First node length of the forth fruiting branch,FNLFFB)、第七果枝第一果节长度(First node length of the seventh fruiting branch,FNLSFB)等5个株型性状和开花期(Flowering time,FT)、花铃期(Flowering to boll-opening period,FBP)、全生育期(Whole growth period,WGP)等3个生育期性状的QTL(Quantitative trait loci,数量性状位点)定位研究。【结果】 8个性状均呈现连续的双向超亲分布,性状之间存在广泛的正向相关性,PH与其他株型性状均为极显著正相关,NFFB与3个生育期相关性状均为显著或极显著正相关。共定位到36个加性QTL(Additive QTL,aQTL)位点,包括14个PH相关aQTL、6个NFFB相关aQTL、3个HNFFB相关aQTL、5个FNLFFB相关aQTL、3个FNLSFB相关aQTL、2个FT相关aQTL、2个FBP相关aQTL、1个WGP相关aQTL,单个aQTL贡献率为1.70%~10.38%。这些aQTL分布于20条染色体,每条染色体有1~4个aQTL。发现3个aQTL重叠区段,分别为A11染色体的qNFFB-A11-1qWGP-A11-1、D3染色体的qHNFFB-D3-1qPH-D3-1、D8染色体的qNFFB-D8-1qFNLSFB-D8-1。检测到263个上位性QTL(Epistatic QTL,eQTL),单个eQTL的贡献率为1.17%~6.19%;19个aQTL与21个eQTL位置重叠;At基因组分布有17个aQTL和202个eQTL,Dt基因组分布有19个aQTL和61个eQTL。【结论】 本研究为探索棉花株型的分子遗传机制奠定了研究基础,为机采棉分子标记辅助育种提供理论指导。

关键词: 陆地棉; 株型; 生育期; 数量性状位点定位

Abstract:

[Objective] The aim of this study is to deeply analyze the molecular genetic mechanism of cotton plant architecture and growth period traits, and to accelerate the process of molecular marker-assisted breeding of machine-picked cotton varieties. [Method] QTL (Quantitative trait loci) mapping was conducted for plant height (PH), node of the first fruiting branch (NFFB), height of NFFB (HNFFB), first node length of the forth fruiting branch(FNLFFB), first node length of the seventh fruiting branch (FNLSFB), flowering time (FT), flowering to boll-opening period (FBP), and whole growth period (WGP), based on a high density genetic map and an F2 population containing 413 plants. [Result] The 8 observed traits showed continuous and bidirectional super affinity distribution. Significant positive correlations were found among the traits. A total of 36 additive effect QTL (aQTL) were mapped, including 14 PH-related aQTL, 6 NFFB-related aQTL, 3 HNFFB-related aQTL, 5 FNLFFB-related aQTL, 3 FNLSFB-related aQTL, 2 FT-related aQTL, 2 FBP-related aQTL, and 1 WGP-related aQTL. Each aQTL could explain 1.70%-10.38% of the phenotypic variation (PV). Three aQTL overlapping regions were found on 3 chromosomes (Chr.), including qNFFB-A11-1 and qWGP-A11-1 on Chr. A11, qHNFFB-D3-1 and qPH-D3-1 on Chr. D3, qNFFB-D8-1 and qFNLSFB-D8-1 on Chr. D8. In addition, 263 epistatic effect QTL (eQTL) were detected, and each eQTL contributed 1.17%-6.19% for PV. And 21 eQTL overlapped with 19 aQTL. A total of 17 aQTL and 202 eQTL distributed on the At genome, and 19 aQTL and 61 eQTL distributed on the Dt genome. [Conclusion] This study laid a foundation for exploring the molecular genetic mechanism of cotton plant architecture and provided theoretical guidance for molecular marker assisted breeding of machine-picked cotton.

Key words: Gossypium hirsutum L.; plant architecture; growth period; QTL mapping