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棉花学报 ›› 2018, Vol. 30 ›› Issue (6): 448-454.doi: 10.11963/1002-7807.jgxdxm.20181121

• 研究与进展 • 上一篇    下一篇

基于重测序开发的InDel标记定位陆地棉矮化突变体

季高翔,何守朴,潘兆娥,龚文芳,贾银华,王立如,王朋朋,耿晓丽,杜雄明*   

  1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳455000
  • 收稿日期:2017-12-22 出版日期:2018-11-15 发布日期:2018-11-15
  • 通讯作者: dxm630723@163.com
  • 作者简介:季高翔(1993―),男,硕士研究生,923185578@qq.com
  • 基金资助:
    国家自然科学基金(31671746)

Localization of a Dwarfing Mutation in Upland Cotton Using InDel Markers Based on Genome Re-Sequencing Data

Ji Gaoxiang, He Shoupu, Pan Zhao’e, Gong Wenfang, Jia Yinhua, Wang Liru, Wang Pengpeng, Geng Xiaoli, Du Xiongming*#br#   

  1. Institute of Cotton Research of the Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2017-12-22 Online:2018-11-15 Published:2018-11-15

摘要: 【目的】利用重测序开发的InDel标记实现陆地棉矮化突变体基因AS98的定位。【方法】利用1个极端矮化材料AS98与其野生型LHF10构建F2群体,根据突变体及其野生型的重测序数据开发InDel标记,并利用新开发的分子标记对矮化性状进行定位。【结果】挑选新开发的位于初步定位区间内的InDel标记114对,用2个材料进行标记多态性检测,筛选出具有多态性的标记20对;利用多态性标记在包含223个单株的F2群体中进行聚合酶链式反应,结合表型把矮化基因AS98定位于染色体D12上,与其最近的分子标记是InDel-50,遗传距离为6.9 cM。【结论】本试验证实了利用重测序数据开发InDel标记,并在陆地棉矮化材料衍生的分离群体中进行基因定位的可行性,为进一步精细定位及分子辅助选择育种提供参考。

关键词: 陆地棉; InDel; 矮化; 定位

Abstract: [Objective] InDel markers that were developed by comparing whole genome re-sequencing data were used to map the AS98 gene of a dwarf mutant of upland cotton (Gossypium hirsutum L.). [Method] An F2 segregation population was constructed from an upland-cotton dwarf mutant (AS98) and its wild type (LHF10), and InDel markers were developed based on genome re-sequencing data from the two parents. The InDel markers and the F2 generation were then used to map the AS98 gene of the dwarf mutant. [Result] A total of 114 InDel markers located in a previous mapping region were screened and used to genotype 223 F2 individuals. Ultimately, phenotypic data and 20 polymorphic primer pairs were used to construct a genetic linkage map. Molecular marker analysis mapped AS98 within a 6.9 cM distance of the marker InDel-50 on chromosome D12. [Conclusion] This study has demonstrated the feasibility of using InDel markers developed from re-sequencing data for gene localization in isolated populations derived from upland cotton dwarf materials. The resulting gene map provides a basis for future fine mapping and molecular-assisted selection breeding.

Key words:  Gossypium hirsutum; InDel; dwarf; mapping

中图分类号: 
  • S562.024:Q343.1+7