基于重测序开发的InDel标记定位陆地棉矮化突变体

doi:10.11963/1002-7807.jgxdxm.20181121

摘要/Abstract

摘要： 【目的】利用重测序开发的InDel标记实现陆地棉矮化突变体基因AS98的定位。【方法】利用1个极端矮化材料AS98与其野生型LHF10构建F2群体，根据突变体及其野生型的重测序数据开发InDel标记，并利用新开发的分子标记对矮化性状进行定位。【结果】挑选新开发的位于初步定位区间内的InDel标记114对，用2个材料进行标记多态性检测，筛选出具有多态性的标记20对；利用多态性标记在包含223个单株的F2群体中进行聚合酶链式反应，结合表型把矮化基因AS98定位于染色体D12上，与其最近的分子标记是InDel-50，遗传距离为6.9 cM。【结论】本试验证实了利用重测序数据开发InDel标记，并在陆地棉矮化材料衍生的分离群体中进行基因定位的可行性，为进一步精细定位及分子辅助选择育种提供参考。

关键词: 陆地棉; InDel; 矮化; 定位

Abstract: [Objective] InDel markers that were developed by comparing whole genome re-sequencing data were used to map the AS98 gene of a dwarf mutant of upland cotton (Gossypium hirsutum L.). [Method] An F2 segregation population was constructed from an upland-cotton dwarf mutant (AS98) and its wild type (LHF10), and InDel markers were developed based on genome re-sequencing data from the two parents. The InDel markers and the F2 generation were then used to map the AS98 gene of the dwarf mutant. [Result] A total of 114 InDel markers located in a previous mapping region were screened and used to genotype 223 F2 individuals. Ultimately, phenotypic data and 20 polymorphic primer pairs were used to construct a genetic linkage map. Molecular marker analysis mapped AS98 within a 6.9 cM distance of the marker InDel-50 on chromosome D12. [Conclusion] This study has demonstrated the feasibility of using InDel markers developed from re-sequencing data for gene localization in isolated populations derived from upland cotton dwarf materials. The resulting gene map provides a basis for future fine mapping and molecular-assisted selection breeding.

Key words: Gossypium hirsutum; InDel; dwarf; mapping

中图分类号:

S562.024：Q343.1+7

季高翔, 何守朴, 潘兆娥, 龚文芳, 贾银华, 王立如, 王朋朋, 耿晓丽, 杜雄明. 基于重测序开发的InDel标记定位陆地棉矮化突变体[J]. 棉花学报, 2018, 30(6): 448-454.

Ji Gaoxiang, He Shoupu, Pan Zhao’e, Gong Wenfang, Jia Yinhua, Wang Liru, Wang Pengpeng, Geng Xiaoli, Du Xiongming. Localization of a Dwarfing Mutation in Upland Cotton Using InDel Markers Based on Genome Re-Sequencing Data[J]. Cotton Science, 2018, 30(6): 448-454.

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链接本文: http://journal.cricaas.com.cn/Jweb_mhxb/CN/10.11963/1002-7807.jgxdxm.20181121

http://journal.cricaas.com.cn/Jweb_mhxb/CN/Y2018/V30/I6/448

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