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棉花学报 ›› 2017, Vol. 29 ›› Issue (5): 415-427.doi: 10.11963/1002-7807.gxllf.20170830

• 研究与进展 • 上一篇    下一篇

棉花BZR基因家族的全基因组鉴定及表达分析

郭新磊1#,路普1#,王园园2,蔡小彦1,王星星1,周忠丽1,王玉红1,王春英1,王坤波1*,刘方1*   

  1. 1. 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000;2. 河南科技学院/现代生物育种河南省协同创新中心,河南 新乡 453003
  • 收稿日期:2016-12-29 出版日期:2017-09-15 发布日期:2017-09-15
  • 通讯作者: liufcri@163.com;王坤波,wkbcri@163.com
  • 作者简介:郭新磊(1989―),男,硕士,guoxlcaas@163.com;路普(1992―),男,硕士研究生,lupu1992@163.com;#同等贡献。
  • 基金资助:
    国家自然科学基金(31671745);国家重点研发计划(2016YFD0101401,2016YFD0100203)

Genome-Wide Identification and Expression Analysis of the Gene Family Encoding Brassinazole Resistant Transcription Factors in Cotton

Guo Xinlei1#, Lu Pu1#, Wang Yuanyuan2, Cai Xiaoyan1, Wang Xingxing1, Zhou Zhongli1, Wang Yuhong1, Wang Chunying1, Wang Kunbo1*, Liu Fang1*   

  1. 1. Institute of Cotton Research of the Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China; 2. Henan Institute of Science and Technology/Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang, Henan 453003, China
  • Received:2016-12-29 Online:2017-09-15 Published:2017-09-15

摘要: 【目的】油菜素唑抗性因子(Brassinazole resistant transcription factor,BZR)是植物油菜素内酯(Brassinosteroid,BR)信号通路中的1类重要转录因子,通过调控相关基因的表达,参与植物生长发育过程。本研究旨在探索棉花中BZR基因的数量及其功能。【方法】利用生物信息学方法,对雷蒙德氏棉、亚洲棉和陆地棉中的BZR基因进行全基因组鉴定及表达模式分析。【结果】雷蒙德氏棉、亚洲棉和陆地棉中分别有7、7和14个BZR基因;通过序列特征分析和系统发育分析可将棉花BZR基因分为2组(a组和b组)。内含子/外显子结构分析发现:大部分BZR基因具有2个外显子,a组成员中均具有较长的内含子序列,b组成员的内含子序列较短,同组BZR成员具有相似的基因结构。基因复制分析表明:片段复制是棉花BZR基因家族扩增的主要方式,棉花BZR基因在进化过程中经历了严格的纯化选择。转录组数据分析表明:14个GhBZR基因在陆地棉的7个组织中特异表达,大多数GhBZR基因在茎、叶、花瓣和雌蕊中高表达;许多GhBZR基因的表达受非生物胁迫诱导。【结论】推测GhBZR基因可能参与棉花抗逆及纤维发育。本研究结果可为棉花BZR基因的生物学功能研究提供信息。

关键词: 棉花; BZR基因; 系统发育分析; 表达分析

Abstract: [Objective] Brassinazole resistant transcription factors (BZR) play important roles in Brassinosteroid (BR) signal transduction in plants. The BZR gene family is involved in plant developmental processes by regulating the expression of related genes. The aim of this study is to characterize the number, evolutionary characteristics, and biological function of BZR genes in cotton. [Method] We conducted genome-wide identification and expression analysis of the BZR gene family in Gossypium raimondii, G. arboreum, and G. hirsutum. [Result] In this study, seven, seven, and 14 BZR genes were identified from G. raimondii, G. arboreum, and G. hirsutum, respectively. These BZR genes were grouped into two groups, a and b, based on their amino acid sequences and phylogenetic analysis. Gene structure analysis showed that most BZR genes contain two exons. Additionally, BZR genes in "a" group had a longer intron than those in "b" group; within the same group, BZR genes had a similar gene structure.  Analysis of gene duplication revealed that segmental duplications played crucial roles in BZR gene family expansion, with cotton BZR genes experiencing purifying selection during evolution. Transcriptome data analysis showed that 14 GhBZRs had differing expression patterns in different organs; most GhBZRs were highly expressed in stem, leaf, petal, and pistil tissue, and were induced or repressed by abiotic stress. [Conclusion] These results suggest that GhBZR genes may be involved in stress resistance and fiber development in cotton. Furthermore, they provide information for further analysis aimed at uncovering the biological functions of BZR genes in cotton.

Key words: cotton; BZR genes; phylogenetic analysis; expression analysis

中图分类号: 
  • S562.01