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棉花学报 ›› 2018, Vol. 30 ›› Issue (6): 492-497.doi: 10.11963/1002-7807.gsylff.20181113

• 研究简报 • 上一篇    下一篇

陆地棉新陆早45号体细胞胚胎发生及再生体系的建立

葛书娅#,沈秋平#,何积明,吕尊富,郑海彪,李飞飞*   

  1. 浙江农林大学农业与食品科学学院,浙江 临安311300
  • 收稿日期:2017-07-21 出版日期:2018-11-15 发布日期:2018-11-15
  • 通讯作者: lifei-fei@163.com
  • 作者简介:葛书娅(1997―),女,在读本科,1017255621@qq.com;#同等贡献
  • 基金资助:
    国家自然科学基金青年基金(31401461);2018浙江省大学生科技创新活动计划暨新苗人才计划项目(2018R412001);2018年国家大学生创新创业训练计划项目(201810341002)

A Regeneration System for Cotton Variety Xinluzao 45 via Somatic Embryogenesis

Ge Shuya#, Shen Qiuping#, He Jiming, Lü Zunfu, Zheng Haibiao, Li Feifei*   

  1. Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang 311300, China
  • Received:2017-07-21 Online:2018-11-15 Published:2018-11-15

摘要: 目的】拓展胚胎发生再生的陆地棉基因型,为棉花遗传转化提供优良受体材料。【方法】以新疆推广的陆地棉品种新陆早45号下胚轴为外植体,设计了6种不同激素组合的培养基进行初生愈伤组织诱导, 建立其胚胎发生的再生体系。【结果】新陆早45号下胚轴在激素组合C1 [0.1 mg·L-1 2,4-D(二氯苯氧乙酸) + 0.1 mg·L-1 KT(呋喃氨基嘌呤)]和C5 [0.1 mg·L-1 2,4-D + 0.5 mg·L-1 IBA(吲哚-3-丁酸)+0.1 mg·L-1 KT]均可诱导出淡黄色疏松状的初生愈伤组织。将增殖后的愈伤组织培养在去掉激素、 KNO3加倍的MS3培养基上可诱导出胚性愈伤组织,实现胚胎发生和植株再生,初生愈伤组织诱导率和分化率最高分别可达到92.2%和40.3%。研究发现C1和C5激素组合的新陆早45号下胚轴出愈率没有显著差异,但C1诱导愈伤组织的胚胎发生率明显优于C5。【结论】成功建立了陆地棉新陆早45号的胚胎发生再生体系,可为下一步通过农杆菌介导转化新陆早45号提供技术支持。

关键词: 陆地棉; 新陆早45号; 下胚轴; 胚性愈伤组织; 再生体系

Abstract: [Objective] The purpose of this study was to expand upland cotton genotypes suitable for regeneration via somatic embryogenesis, so as to provide excellent receptor materials for the genetic transformation of cotton. [Method] Six different hormone combinations were studied to establish a somatic embryo regeneration system for hypocotyl explants of Xinluzao 45, a widely planted variety in Xinjiang Province. [Result] Faint yellow callus was induced on a medium consisting of hormone combinations C1 (0.1 mg·L-1 acetic acid dichlorobenzene oxide [2, 4-D] + 0.1 mg·L-1 kinetin [KT]) or C5 (0.1 mg·L-1 2, 4-D + 0.5 mg·L-1 indo-3-butyric acid + 0.1 mg·L-1 KT). The generated callus was able to successfully induce embryogenic callus on hormone-free medium containing doubled KNO3. The embryogenic callus then developed into somatic embryos and regenerated plants. Maximum rates of callus induction and regeneration were 92.2% and 40.3%, respectively. Although no significant difference was observed in the rate of callus induction of Xinluzao 45 hypocotyls between hormone combinations C1 and C5, the incidence of induced embryonic callus was higher on C1 than on C5. [Conclusion] The somatic embryo regeneration system developed in this study provides technical support that will facilitate Agrobacterium-mediated transformation of upland cotton Xinluzao 45.

Key words: Gossypium hirsutum L.; Xinluzao 45; hypocotyl; embryogenic callus; regeneration system

中图分类号: 
  • S562.03:Q813.1