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棉花学报 ›› 2017, Vol. 29 ›› Issue (4): 385-392.doi: 10.11963/1002-7807.gjyqyy.20170531

• 研究简报 • 上一篇    下一篇

不同处理诱导新海16号体细胞胚胎同步化发生

郭家雁1,张霞1,丁喜莲1,李娟1,邓莉1,陈全家1,孙国清2*,曲延英1*   

  1. 1. 新疆农业大学农学院/农业生物技术重点实验室,乌鲁木齐 830052;2. 中国农业科学院生物技术研究所,北京 100081
  • 收稿日期:2017-01-19 出版日期:2017-07-15 发布日期:2017-07-15
  • 通讯作者: 孙国清,sunguoqing1120@126.com;曲延英,xjyyq5322@126.com
  • 作者简介:孙国清,sunguoqing1120@126.com;曲延英,xjyyq5322@126.com
  • 基金资助:
    国家自然科学基金(31660078);棉花生物学国家重点实验室开放课题基金(CB2015A24)

Synchronized Somatic Embryogenesis of 'Xinhai 16' Induced by Different Treatments

Guo Jiayan1, Zhang Xia1, Ding Xilian1, Li Juan1, Deng Li1, Chen Quanjia1, Sun Guoqing2*, Qu Yanying1*   

  1. 1. College of Agriculture/Key Laboratory of Agricultural Biotechnology, Xinjiang Agricultural University, Urumqi 830052, China; 2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-01-19 Online:2017-07-15 Published:2017-07-15

摘要: 【目的】旨在探索诱导海岛棉体细胞胚胎同步化发生的有效方法。【方法】以海岛棉品种新海16号的胚性愈伤组织为材料,研究了低温、饥饿和渗透处理诱导体细胞胚胎同步化发生的效果。【结果】在低温处理组中,4 ℃诱导时体细胞胚胎发生的数量随着低温诱导时间的延长而显著增加;10 ℃诱导时,不同诱导时间下各处理体细胞胚胎发生的数量差异不显著,但都显著高于对照(28 ℃);缺磷、缺氮、缺肌醇3种饥饿处理的体细胞胚胎的数量都随着处理时间的延长而减少;渗透处理组中,在含40 g·L-1葡萄糖的培养基上诱导7 d的效果最佳。【结论】4 ℃处理3 d、缺磷处理5 d、缺氮处理5 d以及40 g·L-1葡萄糖诱导7 d可以显著促进新海16号的体细胞胚胎同步化发生,其中4 ℃处理3 d的诱导效果最佳。

关键词: 海岛棉; 体细胞胚胎; 诱导; 同步化

Abstract: [Objective] The aim of this study was to develop an efficient method for synchronizing the somatic embryogenesis of Gossypium barbadense L. [Method] We attempted to synchronize the somatic embryogenesis of G. barbadense L. cv. 'Xinhai 16' embryonic calli. The somatic embryos induced by low temperature, starvation, and osmotic pressure were counted and analyzed. [Result] In the low-temperature treatment group, the number of somatic embryos increased significantly at 4 ℃. While, there were no significant differences in the number of somatic embryos between treatment times at 10 ℃. Nevertheless, more somatic embryos were produced at 10 ℃ than at 28 ℃ (control temperature). Somatic embryogenesis was inhibited by the nutrient starvation treatment (i.e., phosphate, nitrogen, or inositol deficiency). In the osmotic stress group, the best results were observed following a 7-day culture in medium containing 40 g·L-1 glucose. [Conclusion] 'Xinhai 16' somatic embryogenesis can be synchronized by incubating calli at 4 ℃ for 3 days, simulating phosphate or nitrogen starvation conditions for 5 days, or maintaining calli in a medium containing 40 g·L-1 glucose for 7 days. The low-temperature treatment may provide optimal synchronization conditions.

Key words: Gossypium barbadense L; somatic embryogenesis; inducement; synchronization

中图分类号: 
  • S562.01