棉花学报 ›› 2021, Vol. 33 ›› Issue (3): 247-257.doi: 10.11963/1002-7807.dwfsyw.20210428
党文芳1,2(),刘萍1,2,管力慧1,2,杨红梅1,3,牛新湘4,6,李萍5,楚敏1,3,娄恺1,3,史应武1,2,3,6,*(
)
收稿日期:
2020-04-07
发布日期:
2021-06-02
通讯作者:
史应武
E-mail:1954358900@qq.com;syw1973@126.com
作者简介:
党文芳(1994―), 基金资助:
Dang Wenfang1,2(),Liu Ping1,2,Guan Lihui1,2,Yang Hongmei1,3,Niu Xinxiang4,6,Li Ping5,Chu Min1,3,Lou Kai1,3,Shi Yingwu1,2,3,6,*(
)
Received:
2020-04-07
Published:
2021-06-02
Contact:
Shi Yingwu
E-mail:1954358900@qq.com;syw1973@126.com
摘要:
【目的】分析实验室条件下土壤理化性质对棉花黄萎病拮抗细菌数量及活性的影响,揭示拮抗细菌在土壤不同理化条件下的适应能力。【方法】以已筛选的4个棉花黄萎病拮抗细菌菌株为基础,采用涂布法测定培养皿土壤中不同理化因子(含水率、温度、含盐量、肥力、pH、土壤类型)下的拮抗细菌数量;通过最适土壤理化环境下的盆栽试验,对棉株根部土壤中的拮抗细菌数量进行测定并对棉花黄萎病发生情况进行统计。【结果】SHZ-24和BHZ-29菌株在土壤中活菌相对数量较高,其中SHZ-24的活菌相对数量在15%的土壤含水率下达到27.74%,BHZ-29菌株在中等肥力、灰漠土中分别达到24.81%和15.24%。在高肥、土壤含水率小于20%的土壤条件下,4种拮抗细菌对棉花黄萎病的防病效果均较好,其中BHZ-29防效最高(87.12%),SMT-24防效最低(35.04%)。【结论】4株拮抗细菌在水分合适和施肥土壤中有很好的适应性,可维持一定数量和活性。
党文芳,刘萍,管力慧,杨红梅,牛新湘,李萍,楚敏,娄恺,史应武. 土壤环境因子对棉花根际与内生拮抗细菌存活数量的影响[J]. 棉花学报, 2021, 33(3): 247-257.
Dang Wenfang,Liu Ping,Guan Lihui,Yang Hongmei,Niu Xinxiang,Li Ping,Chu Min,Lou Kai,Shi Yingwu. Effects of soil environmental factors on the survival of cotton rhizosphere and endophytic antagonistic bacteria[J]. Cotton Science, 2021, 33(3): 247-257.
表1
不同采样地土壤理化性质"
土壤 类型 | 取样地 Sampling site | 养分含量Nutrient content/(g·kg-1) | pH | ||||||
有机质 Organic matter | 全氮 Total nitrogen | 全磷 Total phosphorus | 全钾 Total potassium | 碱解氮 Alkali nitrogen | 速效磷 Available phosphorus | 速效钾 Available potassium | |||
棕钙土 | 博乐Bole | 22.753 | 1.539 | 0.972 | 25.066 | 0.022 | 0.029 | 0.217 | 7.55 |
灰漠土 | 石河子Shihezi | 24.579 | 1.598 | 0.970 | 24.145 | 0.011 | 0.015 | 0.313 | 7.89 |
棕漠土 | 库尔勒Korla | 10.833 | 0.727 | 0.838 | 13.910 | 0.004 | 0.047 | 0.151 | 8.03 |
表2
4株拮抗细菌在不同土壤理化因子下的菌落存活数量方差分析结果"
土壤因子 Soil factor | 拮抗细菌 Antagonistic bacteria | Ⅲ型平方和 Sum of square, Type III | 自由度 Degrees of freedom | 均方 Mean of square | F | 概率 Probability (P) |
土壤含水率 | SHZ-24 | 1.34×1013 | 2 | 6.68×1012 | 10.865 | 0.002 |
Soil moisture content | SMT-24 | 8.53×1011 | 2 | 4.27×1011 | 9.035 | 0.004 |
SHT-15 | 3.21×1012 | 2 | 1.61×1012 | 16.247 | 0.000 | |
BHZ-29 | 2.04×1013 | 2 | 1.02×1013 | 40.933 | 0.000 | |
肥力 | SHZ-24 | 1.71×1015 | 2 | 8.53×1014 | 3 111.006 | 0.000 |
Fertility | SMT-24 | 7.11×1013 | 2 | 3.55×1013 | 2 988.233 | 0.000 |
SHT-15 | 1.42×1013 | 2 | 7.10×1012 | 77.034 | 0.000 | |
BHZ-29 | 2.31×1013 | 2 | 1.15×1013 | 121.524 | 0.000 | |
土壤类型 | SHZ-24 | 5.74×1015 | 3 | 1.91×1015 | 246.498 | 0.000 |
Soil type | SMT-24 | 4.90×1015 | 3 | 1.63×1015 | 64.030 | 0.000 |
SHT-15 | 6.87×1015 | 3 | 2.29×1015 | 490.115 | 0.000 | |
BHZ-29 | 8.04×1015 | 3 | 2.68×1015 | 490.115 | 0.000 | |
pH | SHZ-24 | 1.37×1012 | 2 | 6.86×1011 | 3.210 | 0.076 |
SMT-24 | 3.73×1013 | 2 | 1.87×1013 | 255.465 | 0.000 | |
SHT-15 | 5.31×1012 | 2 | 2.66×1012 | 60.323 | 0.000 | |
BHZ-29 | 2.53×1013 | 2 | 1.27×1013 | 103.773 | 0.000 | |
含盐量 | SHZ-24 | 8.32×1011 | 2 | 4.16×1011 | 0.333 | 0.723 |
Salt content | SMT-24 | 2.47×1012 | 2 | 1.23×1012 | 99.360 | 0.000 |
SHT-15 | 5.45×1012 | 2 | 2.72×1012 | 60.734 | 0.000 | |
BHZ-29 | 3.11×1013 | 2 | 1.56×1013 | 112.275 | 0.000 | |
温度 | SHZ-24 | 2.39×1012 | 2 | 1.20×1012 | 1.803 | 0.207 |
Temperature | SMT-24 | 3.31×1012 | 2 | 1.65×1012 | 118.385 | 0.000 |
SHT-15 | 2.11×1013 | 2 | 1.05×1013 | 225.264 | 0.000 | |
BHZ-29 | 8.30×1012 | 2 | 4.15×1012 | 38.326 | 0.000 |
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