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棉花学报 ›› 2018, Vol. 30 ›› Issue (2): 111-118.doi: 10.11963/1002-7807.dsysx.20180302

• 研究与进展 •    下一篇

ghr-miR160负调控棉花叶片衰老机理研究

段珊1,2,窦玲玲1,2,程帅帅1,2,郭亚宁1,2,庞朝友2,马启峰2,魏恒玲2,范术丽2,王寒涛2,喻树迅1,2*   

  1. 1.西北农林科技大学农学院,陕西 杨陵 712100;2.中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南 安阳 455000
  • 收稿日期:2016-12-01 出版日期:2018-03-15 发布日期:2018-03-15
  • 通讯作者: ysx195311@163.com
  • 作者简介:段珊(1990―),女,硕士研究生,18567729482@163.com。
  • 基金资助:
    国家现代农业产业技术体系棉花产业技术体系(CARS-18-02A)

ghr-miR160, a Class of Gossypium hirsutum L. MicroRNA, Negatively Regulates the Progress of Leaf Senescence

Duan Shan1,2, Dou Lingling1,2, Cheng Shuaishuai1,2, Guo Yaning1,2, Pang Chaoyou2, Ma Qifeng2, Wei Hengling2, Fan Shuli2, Wang Hantao2, Yu Shuxun1,2*   

  1. 1.College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Institute of Cotton Research, Chinese Academy of Agricultural Sciences/ State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China
  • Received:2016-12-01 Online:2018-03-15 Published:2018-03-15

摘要: 【目的】陆地棉(Gossypium hirsutum L.)叶片早衰会导致棉花减产降质。MicroRNA(miRNA)是1类长度约为21 nt、在转录后水平调节基因表达的内源非编码小分子RNA。研究ghr-miR160对叶片衰老的调控具有重要意义。【方法】对ghr-miR160进行生物信息学分析、棉花子叶表达模式分析及过表达载体转化拟南芥验证分析。【结果】对ghr-miR160及pre-miR160结构分析表明,miR160在进化过程中高度保守。qRT-PCR结果显示,ghr-miR160在生长35 d的早熟不早衰品种辽4086子叶中优势表达。农杆菌介导转化拟南芥发现,该基因可使拟南芥出现晚抽薹、晚开花和叶片衰老延迟现象。【结论】ghr-miR160可能对棉花叶片衰老起负调控作用,同时为进一步阐明其作用机制奠定了基础。

关键词: Gossypium hirsutum L.; ghr-miR160; 叶片衰老; 基因表达; 负调控; 生物信息学分析

Abstract: [Objective] For Gossypium hirsutum L., leaf premature senescence leads to reduced yield and quality. MicroRNA (miRNA) is a class of endogenous, non-coding small RNA, about 21 nucleotides in length, which regulates gene expression at the post-transcriptional level. This study aimed to investigate the effect of ghr-miR160 in regulating leaf senescence. [Method] ghr-miR160 bioinformatics analysis, expression pattern analysis in cotton cotyledons, and overexpression experiments in Arabidopsis were conducted. [Result] Multiple sequence alignment and phylogenetic analysis revealed that ghr-miR160 was highly conserved during evolution. Real-time quantitative reverse transcription polymerase chain reaction showed that ghr-miR160 was upregulated in 35-day-old cotyledons of Liao 4086 (early maturity without senescence). The transgenic carrying ghr-miR160 showed delayed leaf senescence, and increased blotting time, leading to late flowering. [Conclusion] These results confirmed that ghr-miR160 could delay leaf senescence, which provided a foundation for elucidating its functional mechanism.

Key words:   Gossypium hirsutum L; ghr-miR160; leaf senescence; gene expression; negative regulation; bioinformatics analysis

中图分类号: 
  • S562.04