ghr-miR160负调控棉花叶片衰老机理研究

doi:10.11963/1002-7807.dsysx.20180302

棉花学报 ›› 2018, Vol. 30 ›› Issue (2): 111-118.doi: 10.11963/1002-7807.dsysx.20180302

• 研究与进展 • 下一篇

ghr-miR160负调控棉花叶片衰老机理研究

段珊^1,2，窦玲玲1^,2，程帅帅^1,2，郭亚宁^1,2，庞朝友²，马启峰²，魏恒玲²，范术丽²，王寒涛²，喻树迅^1,2*

1.西北农林科技大学农学院，陕西杨陵 712100；2.中国农业科学院棉花研究所/棉花生物学国家重点实验室，河南安阳 455000

收稿日期:2016-12-01 出版日期:2018-03-15 发布日期:2018-03-15
通讯作者: ysx195311@163.com
作者简介:段珊（1990―），女，硕士研究生，18567729482@163.com。
基金资助:
国家现代农业产业技术体系棉花产业技术体系（CARS-18-02A）

ghr-miR160, a Class of Gossypium hirsutum L. MicroRNA, Negatively Regulates the Progress of Leaf Senescence

Duan Shan^1,2, Dou Lingling^1,2, Cheng Shuaishuai^1,2, Guo Yaning^1,2, Pang Chaoyou², Ma Qifeng², Wei Hengling², Fan Shuli², Wang Hantao², Yu Shuxun^1,2*

1.College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China; 2. Institute of Cotton Research, Chinese Academy of Agricultural Sciences/ State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China

Received:2016-12-01 Online:2018-03-15 Published:2018-03-15

摘要/Abstract

摘要： 【目的】陆地棉（Gossypium hirsutum L.）叶片早衰会导致棉花减产降质。MicroRNA(miRNA)是1类长度约为21 nt、在转录后水平调节基因表达的内源非编码小分子RNA。研究ghr-miR160对叶片衰老的调控具有重要意义。【方法】对ghr-miR160进行生物信息学分析、棉花子叶表达模式分析及过表达载体转化拟南芥验证分析。【结果】对ghr-miR160及pre-miR160结构分析表明，miR160在进化过程中高度保守。qRT-PCR结果显示，ghr-miR160在生长35 d的早熟不早衰品种辽4086子叶中优势表达。农杆菌介导转化拟南芥发现，该基因可使拟南芥出现晚抽薹、晚开花和叶片衰老延迟现象。【结论】ghr-miR160可能对棉花叶片衰老起负调控作用，同时为进一步阐明其作用机制奠定了基础。

关键词: Gossypium hirsutum L.; ghr-miR160; 叶片衰老; 基因表达; 负调控; 生物信息学分析

Abstract: [Objective] For Gossypium hirsutum L., leaf premature senescence leads to reduced yield and quality. MicroRNA (miRNA) is a class of endogenous, non-coding small RNA, about 21 nucleotides in length, which regulates gene expression at the post-transcriptional level. This study aimed to investigate the effect of ghr-miR160 in regulating leaf senescence. [Method] ghr-miR160 bioinformatics analysis, expression pattern analysis in cotton cotyledons, and overexpression experiments in Arabidopsis were conducted. [Result] Multiple sequence alignment and phylogenetic analysis revealed that ghr-miR160 was highly conserved during evolution. Real-time quantitative reverse transcription polymerase chain reaction showed that ghr-miR160 was upregulated in 35-day-old cotyledons of Liao 4086 (early maturity without senescence). The transgenic carrying ghr-miR160 showed delayed leaf senescence, and increased blotting time, leading to late flowering. [Conclusion] These results confirmed that ghr-miR160 could delay leaf senescence, which provided a foundation for elucidating its functional mechanism.

Key words: Gossypium hirsutum L; ghr-miR160; leaf senescence; gene expression; negative regulation; bioinformatics analysis

中图分类号:

S562.04

段珊, 窦玲玲, 程帅帅, 郭亚宁, 庞朝友, 马启峰, 魏恒玲, 范术丽, 王寒涛, 喻树迅. ghr-miR160负调控棉花叶片衰老机理研究[J]. 棉花学报, 2018, 30(2): 111-118.

Duan Shan, Dou Lingling, Cheng Shuaishuai, Guo Yaning, Pang Chaoyou, Ma Qifeng, Wei Hengling, Fan Shu, Wang Hantao, Yu Shuxun. ghr-miR160, a Class of Gossypium hirsutum L. MicroRNA, Negatively Regulates the Progress of Leaf Senescence[J]. Cotton Science, 2018, 30(2): 111-118.

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ghr-miR160负调控棉花叶片衰老机理研究

ghr-miR160, a Class of Gossypium hirsutum L. MicroRNA, Negatively Regulates the Progress of Leaf Senescence

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