Development of a Biological Complex Seed-Coating Agent to Control Verticillium Wilt
Chen Lihua1, He Pengfei1,3, Yuan Dechao2, Ou Xiaohui2, Wu Yixin2,3, He Yueqiu2,3*
1. Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. Faculty of Agronomy and Biotechnology, YAU, Kunming 650201, China; 3. National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650217, China
Abstract：[Objective] The aim of this study is to make use of the antagonism and growth promoting ability of Bacillus methylophicus strain LH-L3, and add it into the chemical seed-coating agent as an active ingredient. [Method] The optimum formula (agent No.4: 2.5% fludioxonil＋10% thiram＋10% carbofuran＋109 cfu·mL－1 LH-L3) was obtained based on the sterilization effect of four seed coating agents on infected seed. Then the survival ability of the strain in agent No.4 and its shelf life were identified by plate test. Finally, the disease and pest control effects were tested in the greenhouse pot experiment. [Result] The results showed that the agent No. 4 had the best control effect, and 1.1×105 cfu·mL－1 of LH-L3 after storage at room temperature for 6 months. Compared with the control, the formula No. 4 increased 11.54% emergence rate, 18.74% plant type height and 20.91% leaf width, respectively, in the 15th day after emergence, and decreased 65.42% and 48.56% of Verticillium wilt at day 2 and day 5 after the disease occurred. The cotton seeds coated with formula No. 4 reduced 83.07% of plant and 92.33% of leaf damaged by Sylepta derogata, compared with the negative control without seed coating. [Conclusion] Strain LH-L3 and chemical pesticide compound as a seed coat agent is completely feasible. And the developed biological complex seed-coating agent has better control effect on Verticillium wilt and Sylepta derogate.
陈丽华,何鹏飞,袁德超, 等. 一种防治棉花黄萎病的生物复合种衣剂的研制[J]. 棉花学报, 2018, 30(3): 282-290,封三.
Chen Lihua,He Pengfei,Yuan Dechao, et al. Development of a Biological Complex Seed-Coating Agent to Control Verticillium Wilt[J]. Cotton Science, 2018, 30(3): 282-290,封三.
Lei Bin, Li Jin, Duan Liusheng, et al. Effect of seed coating agents on external morphology and ultrastructure of cotton radicles and seedlings under low temperature treatments[J]. Chinese Journal of Agrometeorology, 2017, 38(4): 248-256.<br />
Liu Tong, Shen Yongqiang, Liu Zhen, et al. Control effect of three kinds of seed-coating formulations on the root rot of kidney beans[J]. Plant Protection, 2017, 43(2): 216-219.<br />
Ren Meifeng, Lu Junjiao, Li Daqi, et al. Determination of the toxicity for the larve of blank cutworm and protect the seedling effect by the two kinds of seed coating agents[J]. China Plant Protection, 2017, 37(4): 68-70.<br />
Cui Wenyan, He Pengfei, He Pengjie, et al. Effect of mirobial complex seed-coating agent on germination, seedling biological traits and yield of maize[J]. Journal of Yunnan Agricultural University (Natural Science), 2016, 31(4): 630-636.<br />
Dong Liping. Development and application of a compound suspension agent containing <em>Bacillus amyloliquefaciens</em> B9601-Y2 on canola[D]. Kunming: Yunnan Agricultural University, 2012.<br />
Committee on Standardization of Pesticides in China. Testing method of dispersibility for pesticide: GB/T 32775―2016[S]. Beijing: China Standards Press, 2017.<br />
Committee on Standardization of Pesticides in China. Method for determination of pesticide suspension rate: GB/T 14825―2006[S]. Beijing: China Standards Press, 2007.<br />
Committee on Standardization of Pesticides in China. Method for determination of heat storage stability of pesticide: GB/T 19136―2003[S]. Beijing: China Standards Press, 2003.<br />
Committee on Standardization of Pesticides in China. Method for determination of low temperature stability of pesticide: GB/T 19137―2003[S]. Beijing: China Standards Press, 2003.<br />
Shenyang Chemical Industy Research Institute. Guidelines on drafting standards of suspension concentrates for seed dressing: GB/T 17768―1999[S]. Beijing : China Standards Press, 2000.<br />
[12]
Xue Lei, Xue Quanhong, Chen Qin, et al. Isolation and evaluation of rhizosphere actinomycetes with potential application for biocontrol of Verticillium wilt of cotton[J]. Crop Protection, 2013, 43(1): 231-240.<br />
Sun Yimin, Wu Bingzhi, Duan Wenxue. A new method for control striped flea-beetles in cabbage fields[J]. Heilongjiang Agricultural Science, 2000(1): 32-33.<br />
[14]
Zeng Defang, Wang Fang, Wang Zhen. Preparation and study of a novel, environmentally friendly seed-coating agent for wheat[J]. Communications in Soil Science and Plant Analysis, 2012, 43(10): 1490-1497.<br />
Chen Lihua, Gao Qiuyu, Zhang Jiaheng, et al. Effect of a biological complex seed-coating agent on the germination and seedling growth of four pasture grass[J]. Acta Agrestia Sinica, 2016, 24(1): 231-236.<br />
Hua Rimao, Tang Guilan, Li Xuede, et al. Degradation dynamics and combined effect of several pesticides in tobacco[J]. China Environmental Science, 2003, 23(4): 440-443.<br />
Shi Yongqiang, Feng Zili, Li Zhifang, et al. Control of cotton seedling <em>Rhizoctonia solani</em> disease by 400 g·L<sup>－1</sup> thiram·carboxin suspending formulations for seed coating[J]. China Cotton, 2014, 41(9): 28-30.<br />
SSun Hongwei, Yang Chongliang, Lu Xingbo, et al. Effect of 2.5% fludioxonil FSC on cotton seedling diseases in the field[J]. Modern Agrochemicals, 2006, 5(1): 38-39.<br />
Li Fangzhou, Xin Huihui, Zhou Guangwei, et al. Effects of the seed film coating salicylic acid on the chilling tolerance of cotton seedlings[J]. Cotton Science, 2015, 27(6): 589-594. <br />
Li Fangzhou, Ye Jun. Study on application effects of composite coating with exogenous regulator substances in the dropper cotton field[J]. China Cotton, 2017, 44(4): 11-14.<br />
Lou Shanwei, Zhang Pengzhong, Wang Daguang, et al. Study on the inhibitory effect of cotton Verticillium wilt by seed coating with Bacillus subtilis[J]. China Cotton, 2016, 43(6): 28-30</p>
2018, Vol. 30 
