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棉花学报 ›› 2017, Vol. 29 ›› Issue (6): 570-.doi: 10.11963/1002-7807.cblh.20171115

• 研究与进展 • 上一篇    下一篇

黄萎病对棉花叶片显微结构及光合特性的影响

陈兵1*,王静2*,李天南1, 3,林海1**,韩焕勇1,王方永1,王琼1,马麒1   

  1. 1. 新疆农垦科学院棉花研究所/兵团棉花工程技术研究中心,新疆 石河子 832003;2. 新疆石河子职业技术学院水利建筑工程分院,新疆 石河子 832003;3. 中国农业大学,北京 100081
  • 收稿日期:2016-09-18 出版日期:2017-11-15 发布日期:2017-11-15
  • 通讯作者: xjlinh@126.com.
  • 作者简介:陈兵(1979―),男,博士, zyrcb@126.com。*同等贡献:zyrcb@126.com,jing108@126.com
  • 基金资助:
    国家科技支撑计划(2014BAD09B03-3);新疆农垦科学院引导计划(83YYD201508) ;国家自然科学基金(41161068)

Effects of Verticillum Wilt on Leaf Microstructure, Photosynthesis of Cotton

Chen Bing1*, Wang Jing2*, Li Tiannan1,3, Lin Hai2**, Hang Huanyong1, Wang Fangyong1, Wang Qiong1, Ma Qi1   

  1. 1. Cotton Institute / Proving Ground, Xinjiang Academy Agricultural and Reclamation Science, Shihezi, Xinjiang 832000, China; 2. Institute of Water Conservation and Architectural Engineering, Xinjiang Shihezi Vocational College, Shihezi, Xinjiang 832003, China; 3. China Agricultural University, Beijing 100081, China
  • Received:2016-09-18 Online:2017-11-15 Published:2017-11-15

摘要: 【目的】揭示棉花对黄萎病胁迫的反应特征,探讨黄萎病不同发病程度对叶片显微结构和光合特性的影响。【方法】通过田间调查取样,设置5个发病水平(b0~b4),对棉叶进行光合能力测试并观察叶片显微结构。【结果】随着发病程度加重,棉花叶片逐渐失绿发黄,严重的区域产生坏死斑,并伴有叶缘焦枯上卷,直至干枯死亡。叶片气孔发育畸形,叶肉细胞间隙增大,叶片总厚度、上下表皮厚度、栅栏组织厚度和海绵组织厚度均逐渐减小,且差异显著,棉花叶片总厚度顺序为b0>b1>b2>b3>b4,棉花叶片上表皮厚度大小为b0>b1>b2>b3,下表皮、栅栏组织和海绵组织厚度大小为b0>b1>b2。叶片总厚度,上、下表皮厚度,栅栏组织厚度和海绵组织厚度均与叶片发病程度呈极显著负相关。叶片净光合速率、蒸腾速率、气孔导度逐渐降低,且均与b0达极其著差异,胞间CO2浓度呈现先降低后增加的变化趋势,但与b0的差异均不显著。【结论】黄萎病破坏了棉花叶片内部结构,降低了叶片光合能力。

关键词: 棉花; 黄萎病; 叶片; 显微结构; 光合特性

Abstract: [Objective] The purpose of this study was to determine the change  of microstructure and photosynthetic characteristics in cotton leaves under different disease index levels (b0~b4).[Method] Through investigation and sampling in cotton fields, five treatments were designed based on disease index levels of cotton leaf, and the leaf photosynthesis was tested by WFS-3000 and microstructure was observed with Microscope ECLIPSE Ni-E. [Result] With the increase in disease index levels, cotton leaf gradually lost green color, serious necrosis area occurred, with the leaf withered up till dried to death. The stomata of disease leaf incurred abnormally, increasing intercellular space in mesophyll tissue. Total thickness, upper and lower epidermis thickness, palisade tissue thickness and spongy tissue thickness all decreased significantly among all treatments; the trend showed as followed: total thickness was b0>b1>b2>b3>b4, upper epidermis thickness was b0>b1>b2>b3, lower epidermis thickness, palisade and spongy tissue thickness were consistent as b0>b1>b2. As a whole leaf of total thickness, upper, lower epidermis thickness, palisade and spongy tissue thickness were very significantly negatively correlated with leaf disease index levels, The correlation coefficient reached -0.6. The net photosynthesis rate, transpiration rate, stomata conductance gradually decreased and reached significant different with b0, leaf temperature increased, internal CO2 first went up then down but no significant difference with b0. [Conclusion] The results indicated that Verticillum wilt damaged leaf microstructure and impaired photosynthesis of cotton leaf.

Key words: cotton; Verticillum wilt; leaf; microstructure; photosynthesis character

中图分类号: 
  • S562.03