Cotton Science

Figure/Table detail

Comparison of the transcriptomes between pink flower buds and yellow flower buds in upland cotton

Liu Linqiang, Han Xiao, Yang Lan, Yan Qingdi, Hu Wei, Jin Yuying, Yu Jie, Yang Zhaoen, Li Fuguang

Cotton Science, 2023, 35(4): 259-273. DOI: 10.11963/cs20200102

样本 Sample	重复 Replicate	读长数 Reads count	碱基数 Base pair number	Q20/%	Q30/%	GC含量 GC content/%
W-B1	1	22 117 163	6 624 359 478	98.17	94.60	43.72
	2	35 124 976	10 514 644 150	97.79	93.74	43.91
	3	29 513 100	8 839 511 218	98.10	94.44	43.93
W-A9	1	21 904 129	6 569 404 602	98.05	94.33	43.95
	2	22 679 602	6 797 536 744	98.04	94.38	43.78
	3	23 328 611	6 983 879 314	98.16	94.60	43.63
P-B1	1	27 217 944	8 147 805 794	98.16	94.58	43.87
	2	27 589 616	8 267 632 092	98.03	94.36	43.85
	3	25 701 649	7 698 643 432	98.13	94.55	43.76
P-A9	1	22 816 009	6 836 203 750	98.02	94.25	43.82
	2	23 283 592	6 977 127 962	98.14	94.54	43.85
	3	24 341 737	7 291 542 544	98.15	94.60	43.99

Table 3 Basic information for transcriptome sequencing data

Other figure/table from this article

Fig. 1 Flowers and buds at different development stages of CCRI 24 and Zhongyihong A: Flowers and buds at different development stages of CCRI 24 (upper panel) and Zhongyihong (bottorn panel); B: Flowers of CCRI 24 (right) and Zhongyihong (left), photos were taken at the flowering day; C: The color of petals at different development stages of CCRI 24 (upper panel) and Zhongyihong (bottorn panel).
Table 1 Primers used in this study
Table 2 Anthocyanin content in buds at B1 and A9 stages of CCRI 24 and Zhongyihong
Fig. 2 Correlation analysis between different samples based on gene expression levels 1, 2, and 3 followed W-B1, W-A9, P-B1 and P-A9 indicate three replicates. r is the correlation coefficient.
Fig. 3 Comparison of gene expression profiles between qRT-PCR and RNA-Seq Black bars represent gene expression level detected by qRT-PCR, and gray bars represent TPM of genes by RNA-seq.
Table 4 Numbers of DEG between different sample group
Fig. 4 Venn analysis of DEG in buds of CCRI 24 and Zhongyihong at different developmental stages
Fig. 5 The expression profiles of Gh_A07G083500 and Gh_A07G079800 Statistical analyses have been the Student’s test. ** and **** indicate significance at the 0.01 and 0.000 1 probability level, respectively.
Fig. 6 GO enrichment analysis of DEG A: GO enrichment analysis of up-regulated DEG, B: GO enrichment analysis of down-regulated DEG.
Table 5 Metabolic pathways of up-regulated PEG
Table 6 Metabolic pathways of down-regulated DEG
Fig. 7 Gene expression level involved in the biosynthesis, modification and transport pathway of anthocyanin
Fig. 8 The regulation and transport model of anthocyanin in pink flower The dotted line indicates the genes not enriched in this pathway in this study.

模态框（Modal）标题

Figure/Table detail