Cotton Science

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Expression pattern analysis of five detoxification genes in the growth and development of Thrips tabaci and under emamectin benzoate stress

Xin Miaomiao, Wang Xiaoyun, Ji Jichao, Gao Yue, Luo Junyu, Zhang Yinbao, Liu Jun, Zhang Wenbin, Wang Dong, Chen Liangliang, Patima Wumu’erhan, Cui Jinjie

Cotton Science, 2025, 37(1): 38-49. DOI: 10.11963/cs20240046

质量浓度 Mass concentration/(mg·L^-1)	校正死亡率 Adjusted mortality rate/%
100 50 25 12.5 6.25 3.125	100.00 95.00 77.78 58.82 35.00 15.00

Table 2 Insecticidal effect of emamectin benzoate on T. tabaci

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Table 1 Primer information of CYP450 and GST genes of T. tabaci for PCR
Table 3 Toxicity of emamectin benzoate against T. tabaci
Table 4 Physicochemical properties analysis of CYP450 and GST proteins in T. tabaci
Fig. S1 Domain prediction of 5 proteins
Fig. 1 Phylogenetic tree of the CYP4C1(A), CYP6K1 (B), GST1 (C), GSTX1(D) proteins of T. tabaci and homologous proteins of other species
Fig. 2 The expression levels of CYP450 and GST genes at different developmental stages of T. tabaci L1 is the 1^st instar of larvae; L2 is the 2^nd instar of larvae; P is the pupal stage; D1, D3, D5, D7 and D9 are the 1^st, 3^rd, 5^th, 7^th and 9^th day after adult emergence, respectively. Different lowercase letters indicate significant difference (P < 0.05).
Fig. 3 Effects of emamectin benzoate LC₂₀ stress for 24 h (A) and 48 h (B) on the expression of CYP450 and GST genes in T. tabaci *, ** and *** indicated significant differences at the 0.05, 0.01, and 0.001 probability levels, respectively.

模态框（Modal）标题

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