Cotton Science

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Expression pattern analysis of five detoxification genes in the growth and development of Thrips tabaci and under emamectin benzoate stress

Xin Miaomiao, Wang Xiaoyun, Ji Jichao, Gao Yue, Luo Junyu, Zhang Yinbao, Liu Jun, Zhang Wenbin, Wang Dong, Chen Liangliang, Patima Wumu’erhan, Cui Jinjie

Cotton Science, 2025, 37(1): 38-49. DOI: 10.11963/cs20240046

基因 Gene	引物 Primer	上游引物 Forward primer	下游引物 Reverse primer	用途 Purpose
CYP4C101	CYP4C101	TCGGAATCAACGGATTTGGC	GTCATTGACGGCAACAACCT	ORF扩增 ORF amplification
	q-CYP4C101	AAGAAGCGGACGGAGTTGAA	ATGGACAGCACCTTGAGCAT	表达检测 Gene expression detection
CYP4C102	CYP4C102	CGTCCATTGGGAGTGCTTGA	TCGTCAGACCGCAGGATGA	ORF扩增 ORF amplification
	q-CYP4C102	CAATGCCGTCGTTATCCTCAG	GCTGCTGGTGAGAAGTCCTT	表达检测 Gene expression detection
CYP6K1	CYP6K1	ATGATCGGCTGGACCATCG	ACGAGGGCCATCTTGAGCT	ORF扩增 ORF amplification
	q-CYP6K1	TTCGGCATCCAGAGCAACAG	CGCAGGAAGAAGAGCACCAT	表达检测 Gene expression detection
GST1	GST1	TAGATGGCTGTGATCCTGTA	CTGGAGCTTTGCCTTGAG	ORF扩增 ORF amplification
	q-GST1	CGACAACGCCATCCTCTTCA	TGTCCTCCAGCCTCTTCATCT	表达检测 Gene expression detection
GSTX1	GSTX1	TTAGATGGCTCCAACATACA- GG	AGGATTCTTTGGGCGCTTTG	ORF扩增 ORF amplification
	q-GSTX1	CTATGAACGAGGTGCCGAGTG	ATCCTTGCCACCGAGTCCAA	表达检测 Gene expression detection
GADPH	GADPH	CTCATGGTGGCGATACCTCAA	TGCTGGCGATTGTCAATGTTC	表达检测 Gene expression detection

Table 1 Primer information of CYP450 and GST genes of T. tabaci for PCR

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Table 2 Insecticidal effect of emamectin benzoate on T. tabaci
Table 3 Toxicity of emamectin benzoate against T. tabaci
Table 4 Physicochemical properties analysis of CYP450 and GST proteins in T. tabaci
Fig. S1 Domain prediction of 5 proteins
Fig. 1 Phylogenetic tree of the CYP4C1(A), CYP6K1 (B), GST1 (C), GSTX1(D) proteins of T. tabaci and homologous proteins of other species
Fig. 2 The expression levels of CYP450 and GST genes at different developmental stages of T. tabaci L1 is the 1^st instar of larvae; L2 is the 2^nd instar of larvae; P is the pupal stage; D1, D3, D5, D7 and D9 are the 1^st, 3^rd, 5^th, 7^th and 9^th day after adult emergence, respectively. Different lowercase letters indicate significant difference (P < 0.05).
Fig. 3 Effects of emamectin benzoate LC₂₀ stress for 24 h (A) and 48 h (B) on the expression of CYP450 and GST genes in T. tabaci *, ** and *** indicated significant differences at the 0.05, 0.01, and 0.001 probability levels, respectively.

模态框（Modal）标题

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