棉花GASA基因的鉴定及其特征、表达谱

乔凯凯，马常凯，吕娇艳，张朝军^*，马启峰^*，范术丽^*

背景：GASA (Giberellic Acid Stimulated in Arabidopsis)基因家族在植物激素信号通路、生长发育及胁迫响应扮演着重要的角色。在其它植物中已有大量GASA基因被鉴定。然而，棉花中的这类蛋白却知之甚少。

结果：在本研究中，我们通过全基因组分析在草棉、亚洲棉、雷蒙德氏棉、海岛棉和陆地棉中分别鉴定出19、17、25、33、38个GASA基因，并进行全面的生物信息学及表达分析。根据我们的结果，132个GASA蛋白均具有相似的结构，并通过系统发育树被分为4组。共线性分析表明片段复制是GASA基因家族扩张的主要动力。同时，顺式元件和蛋白相互作用网络分析表明GhGASA蛋白在激素响应中起着重要作用。转录组及实时荧光定量PCR分析显示GhGASA基因在不同器官及非生物胁迫下的表达谱呈多样性，揭示了GhGASA基因可能参与纤维发育及非生物胁迫响应。

结论：本文首次对棉花中的GASA基因进行系统的鉴定和分析，指出GhGASA基因在棉花生长发育中起着重要意义。这些结果为进一步研究棉花GASA基因的功能提供了依据。

TiTle：Identification, characterization, and expression profiles of the GASA genes in cotton

Authors: QIAO Kaikai, MA Changkai, LV Jiaoyan, ZHANG Chaojun, MA Qifeng & FAN Shuli

[Background] GASA (Giberellic Acid Stimulated in Arabidopsis) gene family plays a crucial role in the phytohormone signaling pathway, growth and development, and stress responses in plants. Many GASA homologs have been identified in various plants. Nevertheless, little is known about these proteins in cotton.

[Results]In the current study, we identified 19, 17, 25, 33, and 38 GASA genes via genome-wide analyses of Gossypium herbaceum, G. arboreum, G. raimondii, G. barbadense, and G. hirsutum, respectively, and performed comprehensive bioinformatics and expression analyses. According to our results, 132 GASA proteins shared similar protein structures and were classified into four groups based on the phylogenetic tree. A synteny analysis suggested that segmental duplication was a key driver in the expansion of the GASA gene family. Meanwhile, the cis-element and protein interaction analyses indicated that GhGASA proteins play a significant role in the hormone responses. Transcriptomic and qRT-PCR (Quantitative real time-polymerase chain reaction) analyses revealed diverse expression profiles of the GhGASA genes in different organs under abiotic stresses, indicating that some GhGASA genes possibly participate in fiber development and abiotic-stress responses.

[Conclusions] The GASA genes in cotton were systematically identified and analyzed for the first time in this paper, and it suggested that the GASA genes are important to the development and growth of cotton. These results will support future exploration of the functions of GASA genes in cotton.

Journal of Cotton Research. 2021, 4: 7

https://doi.org/10.1186/s42397-021-00082-8

https://jcottonres.biomedcentral.com/articles/10.1186/s42397-021-00081-9