聚丙烯酰胺凝胶银染技术的优化

孟菲, 蔡小彦, 崔兴雷, 刘方, 王星星, 周忠丽, 王春英, 王玉红, 彭仁海, 王坤波

中国棉花 ›› 2015, Vol. 42 ›› Issue (4) : 12-14. DOI: 10.11963/issn.1000-632X.201504004
研究报告

聚丙烯酰胺凝胶银染技术的优化

  • 孟菲1,蔡小彦1,崔兴雷1,刘方1,王星星1,周忠丽1,王春英1,王玉红1,彭仁海2,王坤波1*
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An Optimized Protocol for Silver Staining of Polyacrylamide Gel

  • Meng Fei, Cai Xiaoyan, Cui Xinglei, Liu Fang, Wang Xingxing, Zhou Zhongli, Wang Chunying, Wang Yuhong, Peng Renhai, Wang Kunbo*
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摘要

通过配制固定液、渗透液和显色液中主要成分的梯度浓度,依次处理凝胶,对比显色效果,从而对银染过程中各个试剂的配方进行了探讨和优化。并通过大量的实验验证,在不影响染色质量的前提下缩短了3种处理液的处理时间,明显提高了实验效率。

关键词

聚丙烯酰胺凝胶电泳 / 银染技术

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孟菲, 蔡小彦, 崔兴雷, 刘方, 王星星, 周忠丽, 王春英, 王玉红, 彭仁海, 王坤波. 聚丙烯酰胺凝胶银染技术的优化[J]. 中国棉花, 2015, 42(4): 12-14. https://doi.org/10.11963/issn.1000-632X.201504004
Meng Fei, Cai Xiaoyan, Cui Xinglei, Liu Fang, Wang Xingxing, Zhou Zhongli, Wang Chunying, Wang Yuhong, Peng Renhai, Wang Kunbo. An Optimized Protocol for Silver Staining of Polyacrylamide Gel[J]. China Cotton, 2015, 42(4): 12-14. https://doi.org/10.11963/issn.1000-632X.201504004

参考文献

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基金

国家高技术研究发展计划“四倍体野生棉遗传连锁图谱构建”(2012AA101108-02-05)

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