Cotton Science

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Optimization of the genetic transformation system of cotton shoot tip

Ge Huanyu, Zuo Dongyun, Wang Qiaolian, He Man, Yu Rui, Cheng Hailiang, Chen Quanjia, Lü Limin, Song Guoli

Cotton Science, 2025, 37(5): 429-438. DOI: 10.11963/cs20250046

Fig. 1 Effects of EDTA concentration (A), EDTA water bath time (B), and bacterial suspension concentration (C) on the transient transformation efficiency of shoot tips Different lowercase letters represent significant differences (P < 0.05).

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Table 1 Dosage of different plant growth regulators （mg·L^-1）
Table 2 Effects of different concentrations of six exogenous additives on the transient transformation efficiency and germination rate of shoot tips
Table 3 Effects of different concentrations of spectinomycin on seedling survival rate and positive rate
Table 4 Germination rate under different treatments with different plant growth regulators
Fig. 2 PCR detection results of transgenic plants M: DL2000 DNA Marker. N: negative control; 1-8: transgenic plants to be tested.
Table S1 Formulations of bacterial suspensions, infection solutions, and culture medium
Fig. S1 Shoot tips with green fluorescence detected by fluorescence microscope

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