Molecular Cloning and Expression Analysis of a Receptor-like Protein Kinase Gene in Upland Cotton

YANG Yu-Wen, HE  Bing, ZHANG Bao-Long, NI Wan-Chao

PDF(948 KB)
PDF(948 KB)
Cotton Science ›› 2011, Vol. 23 ›› Issue (1) : 15-21. DOI: 10.11963/cs110103

Molecular Cloning and Expression Analysis of a Receptor-like Protein Kinase Gene in Upland Cotton

  • YANG Yu-wen, HE Bing, ZHANG Bao-long*, NI Wan-chao
Author information +
History +

Abstract

The cDNA encoding the full length of a receptor-like protein kinase of cotton was cloned through degenerate primers amplification, genome walking and RT-PCR. Sequence analysis showed that introns were absent in the gene. It contains a 2964 bp open reading frame, encodes a deduced protein of 988 amino acid residues, and shows 60% homology to HAESA-like2, which regulates floral organ abscission in Arabidopsis. This gene was tentatively designated as GRLK5.  GRLK5 expresses higher in seed, stem bark, root, and fibre than in other organs. The ABA treatment induce the expression of GRLK5. The over expression vector was constructed by inserting GRLK5 into the pCAMBIA2301, tobacco plants were transformed by co-cultivating leaves method via Agrobacterium mediation.The object gene was verified to have been integrated into the genome of tobacco by PCR.

Keywords

upland cotton (Gossypium hirsutum L.) / receptor-like protein kinases / gene expression

Cite this article

Download Citations
YANG Yu-Wen, HE  Bing, ZHANG Bao-Long, NI Wan-Chao. Molecular Cloning and Expression Analysis of a Receptor-like Protein Kinase Gene in Upland Cotton[J]. Cotton Science, 2011, 23(1): 15-21. https://doi.org/10.11963/cs110103

References

[1] 路 梅,徐传雨,郭卫东.植物LRR类受体蛋白激酶的研究进展
[J].浙江师范大学学报:自然科学版,2006,29(3):322- 325.LU Mei, Xu Chuan-yu, Guo Wei-dong. Advance of researches on leucine-rich repeat receptor-like protein kinases of plants
[J]. Journal of Zhejiang Normal University: Natural Science, 2006, 29(3): 322-325.
[2] 闫 锋,祝传书,庞保平.植物类受体蛋白激酶的研究进展
[J].西北植物学报,2009,29(4):851-858.YAN Feng, Zhu Chuan-shu, Pang Bao-ping. Advance in research of plant receptor-like protein kinases
[J]. Acta Botanica Boreali- Occidentalia Sinica, 2009, 29(4): 851-858.
[3] BUTENKO M A, Vie A K, Brembu T, et al. Plant peptides in signalling: looking for new partners
[J]. Trends in Plant Science, 2009, 14(5): 255-263.
[4] ANNE D, Steven E C. LRR-containing receptors regulating plant development and defense
[J]. Development, 2004, 31: 251-261.
[5] DEYOUNG B J, Clark S E. BAM receptors regulate stem cell specification and organ development through complex interactions with CLAVATA signaling
[J]. Genetics, 2008, 180: 895- 904.
[6] ECKARDT N A. Brassinosteroid perception and signaling: heterodimerization and phosphorylation of receptor-like kinases BRI1 and BAK1
[J]. Plant Cell, 2005, 17(6): 1638-1640.
[7] CHO S K, Larue C T, Chevalier D, et al. Regulation of floral organ abscission in Arabidopsis thaliana
[J]. Proc Natl Acad Sci USA, 2008, 105(40): 15629-15634.
[8] SONG Wen-yuan, Wang Guo-liang, Chen Li-li, et al. A receptor kinase-like protein encoded by the rice disease resistance gene,Xa21
[J]. Science, 1995, 270(5243): 1804-1806.
[9] ROBATZEK S, Chinchilla D, Boller T. Ligand-induced endocytosis of the pattern recognition receptor FLS2 in Arabidopsis
[J].Genes Dev, 2006, 20: 537-542.
[10] SUN Yan, Fokar M, Asami T. Characterization of the brassinosteroid insensitive 1 genes of cotton
[J]. Plant Molecular Biology,2004, 54: 221-232.
[11] LI Yuan-li, Sun Jie, Xia Gui-xian. Cloning and characterization of a gene for an LRR receptor-like protein kinase associated with cotton fiber development
[J]. Mol Genet Genomics, 2005, 273: 217-224.
[12] 王红梅,张献龙,李运海,等.陆地棉黄萎病抗性遗传分析
[J].棉花学报,2004,16(2):84-88.WANG Hong-mei, Zhang Xian-long, Li Yun-hai, et al. Analysis on the inheritance of Verticillium dahliae resistance in G.hirsutum
[J]. Cotton Science, 2004, 16(2): 84-88.  
[13] 杨郁文,张保龙,倪万潮,等.两个棉花几丁质酶基因的克隆与表达分析
[J].棉花学报,2008,20(2):88-93.YANG Yu-wen, Zhang Bao-long, Ni Wan-chao, et al. Molecular cloning and expression analysis of two chitinase in upland cotton
[J]. Cotton Science, 2008, 20(2): 88-93.
[14] HOFMANN K, Stoffel W. TMbase-A database of membrane spanning proteins segments
[J]. Biol Chem Hoppe-Seyler, 1993, 374(47): 166.
[15] EMANUELSSON O, Brunak S, von Heijne G, et al. Locating proteins in the cell using TargetP, SignalP and related tools
[J].Nat Protoc, 2007, 2(4): 953-971.
[16] OSAKABE Y, Maruyama K, Seki M, et al. Leucine-rich repeat receptor-like kinase1 is a key membrane-bound regulator of abscisic acid early signaling in Arabidopsis
[J].Plant Cell,2005,17:1105-1119.
[17] 边鸣镝,邓 川,白忠义.玉米类受体蛋白激酶基因ZmLRRPK1的cDNA克隆与表达分析
[J].中国生物工程杂志,2009,29(7):33-36.BIAN Ming-di, Deng Chuan, Bai Zhong-yi. CDNA cloning of maize protein kinase gene ZmLRRPK1 and its expression analysis
[J]. China Biotechnology, 2009, 29(7): 33-36.
[18] MATSUBAYASHI Y, Ogawa M, Morita A, et al. An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine
[J]. Science, 2002, 296(5572): 1470-1472.
PDF(948 KB)

140

Accesses

0

Citation

Detail

Sections
Recommended

/