Cotton Science

Suppressed expression of GhSAMS gene reduced the Helicoverpa armigera resistance in Gossypium hirsutum

Yang Liyan, Li Fang, Pu Zhe, Wang Chuangyun, Qin Lixia

Cotton Science, 2023, 35(3): 165-172. DOI: 10.11963/cs20230012

引物名称 Primer name	引物序列 Primer sequence	用途 Application
GhSAMS-VIGS-NCF	agtggtctctgtccagtcctGGTGATGCAGGTCTCACC	VIGS载体构建
GhSAMS-VIGS-NCR	ggtctcagcagaccacaagtCACACCAATAGCATAGGAGAC	Vector construction for VIGS
GhSAMS-RT-F	GGTGATGCAGGTCTCACC	RT-PCR
GhSAMS-RT-R	CACACCAATAGCATAGGAGAC
GhSAMS-qRT-F	GGTGATGCAGGTCTCACC	qRT-PCR
GhSAMS-qRT-R	CACACCAATAGCATAGGAGAC
GhUBQ7-F	GAAGGCATTCCACCTGACCAAC	内参基因
GhUBQ7-R	CTTGACCTTCTTCTTCTTGTGCTTG	Reference gene

Table 1 Primer sequences used in this study

Other figure/table from this article

Fig. 1 Expression analysis of GhSAMS gene under the stress of H. armigera Different lowercase letters indicate significant difference (P < 0.05).
Fig. 2 Phenotype and silencing efficiency detection of pTRV2::GhSAMS plants A: Phenotypes of different cotton seedlings 14 days after Agrobacterium infection. B: GhSAMS expression level in cotton leaves by semi-quantitative RT-PCR analysis. GhUBQ7 is the reference gene. WT: uninfected wild type plant, CK: plants infected by Agrobacterium with empty vectors.
Fig. 3 Effects of different cotton leaves on the growth and development of H. armigera Different lowercase letters indicate significant difference (P < 0.05).
Fig. 4 Feeding preference of H. armigera to different cotton leaves
Fig. 5 Damage profile of different cotton leaves after H. armigera attack
Fig. 6 H₂O₂ content in cotton leaves analyzed by DAB staining