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Improved Accuracy of Root Length Density Measurements for a Wheat and Cotton Intercropping System or Monocultures by Combining Root Auger Sampling and Image Analysis Methods
Zhang Siping, Zhang Lizhen, Wang Xiaohui, Zhang Lei, Li Pengcheng, Ma Huijuan, Liu Jingran, Liu Shaodong, Zhao Xinhua
PDF(1137 KB)
PDF(1137 KB)
Improved Accuracy of Root Length Density Measurements for a Wheat and Cotton Intercropping System or Monocultures by Combining Root Auger Sampling and Image Analysis Methods
), Zhang Lizhen2, Wang Xiaohui1, Zhang Lei1, Li Pengcheng1, Ma Huijuan1, Liu Jingran1, Liu Shaodong1,*(), Zhao Xinhua1,*()[Objective] The study objective was to improve the accuracy of root length density measurements for a wheat and cotton intercropping system or monocultures using a method that combines field root auger sampling and computer image analysis. [Method] Following field root auger sampling, root length density was measured using an imaging method (DT-SCAN image analysis software) or a ruler (conventional measurement method). The accuracy of the imaging method was assessed using 3423 pairs of root length density data collected from 2002 to 2013. The consistency between the imaging method data (simulated data) and ruler measurements was assessed according to the root mean square error. [Results] The results of the imaging method were correlated with the ruler measurements, although the imaging method was more accurate. Subsequent analyses revealed that in the wheat and cotton intercropping system or monocultures, the accuracy of the imaging method was higher for wheat monocultures than cotton monocultures. Additionally, the accuracy was higher for cotton monocultures than the wheat and cotton intercropping system. Furthermore, the accuracy of the imaging method improved as increasing depths. For the intercropping of wheat and cotton, the accuracy of the imaging method improved from wheat to cotton rows. More accurate data showed: the imaging method accuracy of the wheat rows were higher than the between-wheat rows for wheat monocultures, and that of the between-cotton rows were higher than the cotton rows in cotton monocultures. Regarding the influence of cotton growth periods, the imaging method data for cotton monocultures were more accurate than the data for the wheat and cotton intercropping system before flowering. However, the opposite trend was observed after flowering. [Conclusion] The accuracy of the root length density measurements for the wheat and cotton intercropping system or monocultures increased by combining field root auger samples and an imaging method. This improved method is inexpensive, fast, and enables more accurate analyses of fine lateral roots.
DT-SCAN software / ruler method / root length density / accuracy {{custom_keyword}} /
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