Monitoring of Cry1A Expression and Efficacy to Cotton Bollworm in Bt Cotton Varieties Planted in Hebei, Shandong and Henan Provinces

吕Li-Min , LUO Jun-Yu, LIU Quan-Yi, YANG Zi-Shan, ZHANG  Shuai, WANG Chun-Yi, CUI Jin-Jie

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Cotton Science ›› 2013, Vol. 25 ›› Issue (5) : 459-466. DOI: 10.11963/cs130513

Monitoring of Cry1A Expression and Efficacy to Cotton Bollworm in Bt Cotton Varieties Planted in Hebei, Shandong and Henan Provinces

  • LYU Li-min, LUO Jun-yu, LIU Quan-yi, YANG Zi-shan, ZHANG Shuai, WANG Chun-yi, CUI Jin-jie*
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Abstract

From 2008 to 2012, using the methods of field monitoring, bioassay, expression of Bt exogenous proteins, we detected 129 Bt cotton varieties at 44 plant cotton sections in Hebei, Shandong and Henan cotton region. The field monitoring revealed that No. of remnant cotton bollworm per 100 plants was 20 for the second generation cotton bollworm in 2011, over the control index, but that of other years lower than the control index;  the rate of injured cotton stem-tips, buds and bolls were at a low level. The results of the bioassay showed that the corrected mortality for the second generation bollworm increased at first but then decreased, and that of the third and fourth generation bollworm showed a decreasing tendency. The Bt exogenous protein assays showed expression was decreasing year by year at the seedling stage, whereas the expression of Bt exogenous protein in the budding and bolling stage were increasing a little, but lower than those in seedling stage.

Keywords

Bt cotton / resistance monitoring / population dynamics

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吕Li-Min , LUO Jun-Yu, LIU Quan-Yi, YANG Zi-Shan, ZHANG  Shuai, WANG Chun-Yi, CUI Jin-Jie. Monitoring of Cry1A Expression and Efficacy to Cotton Bollworm in Bt Cotton Varieties Planted in Hebei, Shandong and Henan Provinces[J]. Cotton Science, 2013, 25(5): 459-466. https://doi.org/10.11963/cs130513

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