Progress on Transformation in Fungi Mediated by Agrobacterium tumefaciens

ZHOU Fang-Fang, LI Zhi-Fang, FENG Zi-Li, SHI Yong-Qiang, ZHAO Li-Hong, LI Cai-Hong, ZHU He-Qin

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Cotton Science ›› 2012, Vol. 24 ›› Issue (5) : 461-467. DOI: 10.11963/cs120512

Progress on Transformation in Fungi Mediated by Agrobacterium tumefaciens

  • ZHOU Fang-fang, LI Zhi-fang, FENG Zi-li, SHI Yong-qiang, ZHAO Li-hong, LI Cai-hong, ZHU He-qin*
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Abstract

Agrobacterium tumefaciens-mediated transformation(ATMT) has become an important tool for strain improvement, new genes' tagging and target gene's screening, isolating and cloning. The advantages of this method are simple operation, a wide host range, highly efficient transformation, and a high frequency of single inserted T-DNA. Mutant libraries of many fungi with high capacity and quality have been constructed by ATMT. The screening and identification of related functional genes will be the following research focus on the basis of these mutant libraries. This research will provide a basis for further studies on pathogenic mechanisms and will aid disease-resistance breeding.

Keywords

Agrobacterium tumefaciens / fungus / genetic / transformation / T-DNA insertion / mutants

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ZHOU Fang-Fang, LI Zhi-Fang, FENG Zi-Li, SHI Yong-Qiang, ZHAO Li-Hong, LI Cai-Hong, ZHU He-Qin. Progress on Transformation in Fungi Mediated by Agrobacterium tumefaciens[J]. Cotton Science, 2012, 24(5): 461-467. https://doi.org/10.11963/cs120512

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