Genome-Wide Identification of PPO Gene Family Members and Their Response to Verticillium dahliae in Upland Cotton

Zhang Hui, Tian Xinquan, Gao Wei, Cai Yingfan, Long Lu

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Cotton Science ›› 2017, Vol. 29 ›› Issue (5) : 428-436. DOI: 10.11963/1002-7807.zhll.20170727

Genome-Wide Identification of PPO Gene Family Members and Their Response to Verticillium dahliae in Upland Cotton

  • Zhang Hui, Tian Xinquan, Gao Wei, Cai Yingfan, Long Lu*
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Abstract

[Objective] Polyphenol oxidases (PPOs) are ubiquitous among plants and have been shown to be involved in defense responses to biotic stresses, such as pathogens and insects. The aim of this study was to verify PPO genes and their expression patterns in upland cotton and determine the relationship between PPO and cotton resistance to Verticillium dahliae. [Method] We analyzed changes in PPO activity in allotetraploid cotton TM-1 roots after V991 (V. dahliae) inoculation, and isolated related genes from the TM-1 genome database. Bioinformatics and expression analysis were used to characterize the isolated genes. [Result] 13 putative PPO genes were isolated, all of which were intron-free and encoded proteins containing two copper ion binding sites and conserved PPO domains (tyrosinase, DWL and KFDV domain). Phylogenetic analysis revealed that there was greater genetic diversity in PPO genes within species than between species. GhPPO genes were duplicated, most of which were lowly expressed in all tissues examined, only a few showed tissue-specific expression in different organs. Quantitative real-time polymerase chain reaction showed that GhPPO6D was highly expressed in cotton roots and induced by V. dahliae inoculation. [Conclusion] These results indicated that GhPPO6D may participate in the interaction between cotton and V. dahliae and could be responsible for the up-regulation of PPO activity after V991 infection.

Keywords

Gossypium hirsutum / polyphenol oxidase / gene family / Verticillium dahliae / bioinformatics analysis

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Zhang Hui, Tian Xinquan, Gao Wei, Cai Yingfan, Long Lu. Genome-Wide Identification of PPO Gene Family Members and Their Response to Verticillium dahliae in Upland Cotton[J]. Cotton Science, 2017, 29(5): 428-436. https://doi.org/10.11963/1002-7807.zhll.20170727

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