15 March 2008, Volume 20 Issue 2
    

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  • WANG Jiang-Lin, HU Zheng-Guo, GUO Xiao-Ping, GAO Min, NIE Yi-Chun, ZHANG Xian-Long
    Cotton Science. 2008, 20(2): 83-87.
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    For investigating the cytoplasmic effects of the new type CMS-D8 on yield characters and fiber qualities, three types of crosses were designed by A×R,B×R,R×B, and three male-sterile lines(A line),three corresponding maintainer lines(B line) and three restorer line(R line). A lines and R lines were the fifth generations of backcross successively by domestic materials, and the R lines were crossed to the second generations after backcross. The field planting followed by NCⅡ design with two rows and three replications. The field management and pest management followed essentially the practice under the normal agricultural conditions. The examination of field characters included weight and number of boll, lint percentage, seed yield, and lint yield. The fiber qualities were mainly checked on fiber length, strength, uniformity, elongation, and micronaire.The results showed that the greatly significant negative effects were observed on seed yield and lint yield for sterile cytoplasm of D8, the average seed-lint  yield declined by 15.3%~25.6%,lint yield declined 25.0%~39%;But CMS-D8 had different effects to different yield components, the average decline of bolls weight came to 0.89~1.00 g, the average decline of lint percentage wene 4.59~7.08, but had no significantly on number of boll. The fiber length and fiber uniformity were not influenced by sterile cytoplasm of D8, and the fiber strength was improved by 1.0~1.1 cN·tex-1, micronaire was reduced to 1.4~1.5, fiber elongation was enhanced to  0.4. Different genotypes were influenced differently by sterile cytoplasm of D8.
  • YANG Yu-Wen, ZHANG Bao-Long, NI Wan-Chao, SHEN Xin-Lian, ZHANG Xiang-Gui, XU Ying-Jun
    Cotton Science. 2008, 20(2): 88-93.
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    ESTs encoding class Ⅲ and classⅣ chitinase respectively were isolated from the SSH library inoculated with Verticillium wilt. Both of them contained full 3’ end and their full open reading frame were obtained by RACE and RT-PCR. As a result, the entire coding region of class Ⅲ and classⅣ chitinase is 1390 bp and 678 bp respectively and encodes a polypeptide of 298 and 226 amino acids. The polypeptide encoded by these two genes shared 71% and 65% homology to their homologous proteins of Arabidopsis. They were designated as Ghachi3 and Ghachi4. All of these two genes have signal peptide and were predicted to be extracellular chitinase. Ghachi3 were abundant in petals, buds and phloem, whereas Ghachi4 only showed an extremely high level in phloem by semiquantitative RTPCR. The accumulation of Ghachi4 increased remarkably after treatment of Verticillium wilt and Fusarium wilt in the disease resistant cultivar exclusively. But Ghachi3 was also induced by Verticillium wilt in the susceptible cultivar. In addition, the expression of both the two genes increased remarkably after treatment of ABA. All the results indicated that these two genes might play roles in response to biotic and abiotic stresses.
  • YUE Jie-Yu, WU Li-Jun, WU Yue-Jin, ZHANG Bao-Long, TANG Can-Ming
    Cotton Science. 2008, 20(2): 94-98.
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    The objective of this study was demonstrating nuclei DNA in the pollen grains of upland cotton (Gossypium hirsutum cv. Sumian 22 ). The procedures of detaching the exine and observing nuclei DNA of pollen grains were established. The pollen grains were fixed with 4% paraformaldehyde solution, oxidated with 10% sodium hypochlorite solution, heat shocked at 55℃ for 30 minutes,then the slide with pollens solution was pressed by hand. Finally, the exine of pollen grains could be detached. The pollen grains and de-exined pollen grains were stained with DAPI and observed with fluorescence microscope and laser scanning confocal microscope (Leica TCS SP2), respectively. The nucleus of cotton pollen grain could not be observed, but the nuclear DNA of de-exined pollen grain could be observed clearly. The inner wall fluorescence of pollen grain was not found. The three nuclei of the pollen grain of upland cotton cultivar Sumian 22 were observed in the experiment.
  • YU Hai-Chuan, WU Jiao, CUI Bai-Ming, SUN Jian-Bo, PENG Ming
    Cotton Science. 2008, 20(2): 99-104.
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    In the attempt to elucidate the pivotal function of GA signaling in the initiation and elongation of cotton fiber, two new F-box protein genes were isolated from Gossypium hirsutum for the first time using electronic cloning and RT-PCR methods. They were named GhFB1 and GhFB2, respectively and were submitted to NCBI and the GenBank accession were EF419428 and EF503623. The cDNA of GhFB1 contained 866 bp and encoded a putative protein of 162 aa, while GhFB2 was 920 bp in length, encoding a predicted protein of 161 aa. The encoding proteins have the typical F-box motif of F-box protein family. Bioinformatics analysis indicated that the identities of the deduced GhFB1 and GhFB2 amino acid with Oryza sativa protein, OsGID2, and Arabidopsis thaliana protein, AtSLY1, are very high. Sequence analysis indicated that these two genes are new members of the F-box protein gene family. The expression patterns of GhFB1 and GhFB2 was analyzed by RT-PCR in cotton. It was demonstrated that these two F-box protein genes were both expressed in root, hypocotyls, leaf, stem and fibers, and the highest level of transcripts was to accumulate in the fibers at the stage of initiation and elongation, suggesting that the two F-box genes, GhFB1 and GhFB2, might play important roles in the early stage of fiber development.
  • TU Ye-Gou, WU Kong-Ming, XUE Fang-Sen, Guo-Yu-Yuan
    Cotton Science. 2008, 20(2): 105-109.
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    Effects of host plants on larval developmental duration,pupal weight,fecundity and flight ability of the common cutworm Spodoptera litura, were investigated in the laboratory. The experimental results indicated that there were significant differences in larval developmental duration and survival rate, pupal weight and adult eclosion while the larvae fed on different host crops. The individuals that fed on the artificial diet presented a larval developmental duration of 18.11 d, which was significantly shorter than those on cabbage (20.13 d), soybean (20.45 d), and cotton (20.76 d). The larval survival rates on the artificial diet (91.80%) were significantly higher than thse on cotton \{(75.47%)\}, cabbage (73.68%) and soybean (72.22%). However, there were no significant differences in the adult longevity and pre-oviposition period among the four treatments. The fecundity for the adult from the artificial diet reached to 2141 eggs per female, which was significantly higher than these on cabbage (1879.5 eggs), cotton (1855 eggs), and soybean (1789.5 eggs). Based on a 15-hour tethered-flight test of the adults, moths from the larvae reared on the cabbage could have a much stronger flight than those from the soybean and the cotton,  although it was slower than that from the artificial diet treatment. For the three-days-old moths,the adults from the larval reared on the cabbage could fly 6.77 h and 29.0 2 km, which were significantly higher than those from the soybean by 4.88 h and 18.58 km, and from the cotton by 5.18 h and 18.97 km. These results suggest that the moths from the best host plants in larval stage possess stronger flight ability than those from the poor hosts and migratory activity of the pest, a direct behavior response of the adults to poor environment.
  • ZHANG Hai-Ping, WANG Xue-De, SHAO Ming-Yan, YUAN Shu-Na, LI Xiao
    Cotton Science. 2008, 20(2): 110-115.
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    In order to improve cotton fiber quality, the foreign cellulose synthase genes (acsA and acsB) which were from Acetobacter xylinum, driven by CaMV35S promoter, were introduced into brown color cotton G007, and white color cotton X003, by two means of pollen tube-mediated transformation (ovary injection and pollen grain mediation). Ovary injection, compared to pollen grain mediation, resulted in higher transformation frequencies. Intergration of acsA and acsB genes was confirmed by PCR and southern blot, the result indicated that 11 plants possessed acsA and acsB genes. Four plants with better agronomic traits and fiber quality were selected from transformants. Analysis of transgenic plants and their progenies indicated that some of their fiber properties were improved. The length, strength, cellulose content and lint percent of brown color cotton fiber were increased significantly. However, for white color cotton, although the strength and cellulose content of fiber were increased significantly, the length and lint percent weren’t changed. The fact suggested that induction and expression of acsA and acsB improved fiber quality of transgenic cotton plants.
  • ZHAO Wen-Qing, REN Xiao-Ming, ZHANG Li-Juan, MENG Ya-Li, WANG You-Hua, CHEN Bing-Lin, Zhou-Zhi-Guo
    Cotton Science. 2008, 20(2): 116-122.
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    Cotton fiber length, strength and micronaire are the main quality characters determined by cultivar characteristics, environment and cultivation, and could be used to making sure cotton fiber quality. The objective of this research was to determine the effects of meteorology on fiber properties of cotton and found key meteorologic factorsbased model of fiber length, strength and micronaire. Experiments were random block design with three replications with different cultivars and conducted in different places. Data was collected from 1994—1995 in Shanxi and Shandong province which use three cultivars,and from 2001—2002 in 37 places including cotton variety test region of the Yangtze river, Shanxi and Shandong province which involves 20 cultivars. All experiments are the same in treatments so that experimental errors of soil and environment can be reduced. Based on known research achievement in relationship of cotton fiber quality and cultivars and meteorologic factors, by analyzing experimental data above, the key meteorology factors which significantly affected fiber length, strength, micronaire was determined. The results showed that fiber length was significantly affected by daily average minimum temperature during bolling stage, and so as fiber strength was daily average maximum temperature and relative humidity, and fiber micronaire was daily night temperature and daily average precipitation, respectively. Then, key meteorologic factors-based models of fiber length, strength and micronaire were set up, respectively. These non-liner models calculate both quality of cultivar variables (fiber length, strength, micronaire) and environmental variables (temperature, solar radiation, relative humidity). The RMSE (Root Mean Square Error) of key meteorologic factors-based models of fiber length, strength and micronaire were 0.985 mm, 1.003 cN·tex-1 and 0.233, respectively. Meanwhile, the 1:1 figs of simulated and observed fit well. All results showed that the models could make good prediction. Furthermore, compared to existing models, these models was proved the best in not only emphasizing conclusive effects of cultivar characters but also emboding influence of meteorologic factors on the main cotton fiber quality characters. Finally, the conceptual limits of the models are discussed.
  • LIU Lian-Tao, LI Cun-Dong, SUN Hong-Chun, ZHANG Hai-Na, FENG Li-Xiao
    Cotton Science. 2008, 20(2): 123-127.
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    In recent years, with the generalization of transgenic cotton, premature senescence is aggravated, which has become a main factor for the production of cotton. Characteristics of leaf senescence for main stem of different positions were studied in the later full boll stage of cotton in the field. With Bt-transgenic cotton 33B as experimental plant, by researching into the change trends of contents of chlorophyll and MDA in functional leaves on main stem in the whole growing period, the turning point from blooming to senescence was found in 2005. Hereby in 2006 the physiological characteristics (Chlorophyll, Protein, MDA, SOD, POD) of all leaves at the different stem positions on the cotton plant were researched into in the turning period, and then the differences in senescence between leaves were analyzed. The results showed, (1) The turning point from blooming to senescence is at the later full bolling stage; (2) The Chl content, Pro content and SOD activity decreased with the change of leaf position from stem top to bottom, while the MDA content and POD activity increased; (3) All of the main stem leaves can be separated into three parts on the basis of physiological index changes: bloom leaves (the first leaf and the second leaf on the top), light senescence leaves (the third leaf to the eighth leaf ), heavy senescence leaves (the ninth leaf and the tenth leaf), (4) The middle leaves (light senescence leaves) should be mainly diagnosed and regulated at the full bolling stage, which can postpone the leaf senescence, even the whole plant senescence.
  • WANG Yong-Hui, YANG Chao-Hua, ZHANG Xiang, ZHANG Li, WANG Shu-Hong, WANG Jin-You, CHEN De-Hua
    Cotton Science. 2008, 20(2): 128-132.
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    The growth and development and photosynthetic production characteristics of Bacillus thuringiensis (Bt) transgenic cotton cultivars in transplanting pattern under high fertility, during the 2003 and 2004 growing seasons at the Yangzhou University Farm, Yangzhou of Jiangsu Province, China. The results showed that Bt transgenic hybrid cotton (CCRI 29, Lumian 15 and Kemian 3) had higher contents of chlorophyll, soluble sugar, higher net photosynthetic rate, reproductive organs dry matter weight, and greater intensity for boll setting, enzyme activity of Sucroase from peak square to peak boll period. They had strong photosynthetic production in whole period. The cultivars of GK 19 and GK 99088 had higher contents of chlorophyll, soluble sugar, higher net photosynthetic rate before peak flower period; the cultivars (Kemian 4 and Lumian 22) had higher contents of chlorophyll, soluble sugar, higher net photosynthetic rate after peak flower period; the cultivars of sGK 321 and Lumian 16 had lower contents of chlorophyll, lower net photosynthetic rate in whole period. There were significant correlation between Sucroase activity and reproductive organs dry matter weight, bollsetting intensity, respectively (r=0.9482**,r=0.8699**). These results suggested that appropriate cultivating measures should be adopted concerning with different photosynthetic production characteristics of Bt cotton cultivars, which contributed to bolster the expression of photosynthetic production and improve yield.
  • ZHANG Xiao-Jun, TIAN Xin-Hui, LI Ming-Yue, LIU Hai-Feng, LI Shao-Kun, SONG Wu, SUN Jie
    Cotton Science. 2008, 20(2): 133-136.
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    Pigment in the mature fiber of green fiber and brown fiber defatted with the petroleum ether was extracted by five kinds of neutral solution (chloroform,ethyl acetate,ethyl acetate–ethanol(1:1), ethanol and distilled water). The result showed that the extraction of pigment in natural green fiber by using ethyl acetate–ethanol (1:1), while that in natural brown fiber by using distilled water was the most effective, respectively. The UV spectrum characteristic of the pigment of natural colored cotton was analyzed. There were three absorbance peaks for green pigment at 252 nm, 300 nm and 328 nm, respectively. There was a linear relationship between the absorbance value and its relative concentration at 300 nm or 328 nm. The brown pigment from distilled water distillation had a maximum absorbance peak at 237 nm, but the spectrum of absorbance peak moved to shortwave with the pigment concentration’s declined.
  • FAN Wan-Fa, XING Hong-Yi, HE Dao-Hua, YANG An-Min, ZUO Tian-Fu
    Cotton Science. 2008, 20(2): 137-140.
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    The male sterile line, temporarily designated as Wnafstu developed by NWSUAF, was characterized through several successive backcrossing(plant to plant)using five accessions of G. hirsutum Cv. CCRI 41, Shaan 7359, Shaan 2089, Shaan 2234 and Shaan 204  as recurrent male parents, and crossed with three male-fertile lines (8046-1,8046-2 and 8046-3) with the same genetic background as Wnafstu. The progenies of Wnafstu backcrossed with five accessions were stable in male sterility with 100% plants; but the F1 population of crosses with 8046 was partially fertile with irregular segregation ratio. It inferred that the male sterility was controlled by cytoplasmic genetic factor(s).
  • ZHANG Chao-Jun, LI Fu-Guang, ZHANG Ling
    Cotton Science. 2008, 20(2): 141-147.
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    Induction of embryogenic callus (EC) is a key course of the whole plant regeneration process of plant tissue culture. Recent progresses in plant somatic embryogenesis, including the dynamic change of amino acid, enzyme and phyto hormones, protein marker and gene different expressions, and the heritability of regeneration capacity in vitro plant were reviewed. In the process of inducing callus to EC by tissue culture, the changes would happen to protein components, enzyme activity, phyto-hormone contents with cell functions transforming, and a series of genes expressing in plant, and how these affect the induction were the hot point of tissue culture researches. The research results of the increased content of nucleic acid, amino acid, and protein of EC were applied into the improvement of culture medium which was added more amino acids like Arg and Glu. It was proved that POD, APase and Esterase Isoenzymes played important roles in the differentiation of EC. In the same time, High ABA among of phyto-hormone was benefit to increase the ability of EC differentiation with low PEP. The study on differentia expression of gene was just at the initial stage, in which the common sequencings of protein of 35~55 kD was found as the marker of EC. The differentia expression gene had never been cloned. The researches on proteomics, which should be enhanced, were so far at initial research period of isoenzyme and protein marker. In genetics researches, though the genotype limitation existed in most of plants, the materials with high differentiation rates were only selected in few crops. So it is necessary to further search for the main genes of embryogenic callus and to select the high differentiation materials rapidly.
  • TANG Hai-Ming, CHEN Jin-Xiang
    Cotton Science. 2008, 20(2): 148-150.
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    The effects of floating nursing seedling in water-bed(FNSW) on the cotton yield components and physiological-biochemical characteristics of transplants in the field were performed by using cultivar Nongza 66 as material. The results showed that the average plant height of cotton transplants was 9.1 cm higher, two to three more leaves grew on the main stem, two to three more fruiting branches developed, boll retention was 23.5% added, while the squares and bolls shedding were 3.4% reduced, compared with the control. The analysis of physiological and biochemical traits revealed that the activity of root system, the leaf chlorophyll, soluble protein and sugar content in cotton transplants were increased slightly by FNSW.
  • ZHOU Gui-Sheng, FENG Chao-Nian, XIE Yi-Ming, ZHANG Wang-Ding, YU Jian-Ping, CHEN Gang
    Cotton Science. 2008, 20(2): 151-153.
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    To investigate the effects of temperature and light on fiber quality in Kemian 1, experiments were carried at four different ecological sites in Jiangsu Province. The results indicated that temperature and light at four sites varied significantly. Further regressive analyses indicated that there were different dominant factors affecting different fiber indexes in four types of bolls. The dominant factor affecting fiber length is daily temperature difference. Fiber strength of pre-summer bolls and early autumn bolls was dominantly affected by maximum temperature, while the fiber strength of summer bolls was mainly influenced by daily sunshine duration and daily temperature difference. As for the fiber strength of late autumn bolls, it was mainly influenced by maximum temperature and daily temperature difference. The micronaire readings of fiber in pre-summer bolls were mainly affected by minimum temperature and daily average temperature. The micronaire readings of fiber in summer bolls and early autumn bolls were primarily affected by daily sunshine duration. The micronaire readings of fiber in late autumn bolls were mainly affected by daily temperature difference.
  • FAN Jun-Hua, LIU Ming, CHEN Zhi-Lin
    Cotton Science. 2008, 20(2): 154-157.
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    By measuring the dry matter accumulation,distribution,and the physiological factors of boll shell during the cotton-boll development process of five kinds of island cotton, we get the results:(1)The dry matter in boll shell increases positively with the age of the cotton-boll,then drops quickly,the dry matter accumulation in seed and seed-cotton keeps a positive rise;(2)The ratio of boll shell in the boll decreases gradually , the ratio of seed and seed-cotton increase step by step , reaching their peaks at the end, while the ratio of pure-cotton exhibits a phenomenon of firstly decrease then increase;(3)The content of photosynthetic pigment goes up with the aging of the cotton-boll, while the content of carotenoid, soluble protein and the activity of invertase goes down, indwidudly. Several suggestions are proposed.
  • HOU Jing, SHENG Jian-Dong, Li-Xue-Ni, CHEN Bo-Lang
    Cotton Science. 2008, 20(2): 158-封三.
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    River sand pot experiment was conducted to study screening of the potassium high efficiency among 47 cotton varieties. The result indicated that there were great difference among cotton varieties in the low  potassium ability, cutivars 602, Xinluzao 6, Xinluzhong 15, Xinhai 16, JiaoMian, 183, and Xinhai 13 had higher K utilization efficiency, stronger K absorption ability, and higher K utilization index in low K level; while cutivars ShiK 7, Xinluzao 10 and Xinhai 14  had lower K efficiency , fewer K absorption ability and lower K utilization index in low K level.