ÃÞ »¨ ѧ ±¨      Cotton Science    2008£¬20(5)£º330-334

 

Preliminary Study on the Whole Proteins of Ovule and Water-Soluble Proteins of Seeds in Cotton Fiber Mutants by SDS-PAGE
LAI Tong-fei£¬JIA Yin-hua£¬DU Xiong-ming*
(Cotton Research Institute of CAAS ; Key Laboratory for Cotton Genetic Improvement , Ministry of Agriculture , Anyang , Henan 455000, China)

Abstract£ºThe whole proteins of ovules and water-soluble proteins from seed in cotton fiber mutant were extracted and the variation of the protein contents of cotton ovules during different growing stage were analyzed. Through comparing the SDS-PAGE patterns between the wild types and their mutants, three distinct bands which are probably relatived to the initiation of the ovule epidermal cell have been found between the mutant and its wild type of the diploid cotton.Moreover, we also discovered five distinct bands in the wild type and its mutant of the species from SDS-PAGE profile of seed water-soluble proteins. This research provides some information for further detecting the molecular mechanics about the differentiation and development of cotton fiber.
Key words£ºcotton fiber£»protein£»SDS-PAGE     [Full Text,1653KB¡¢Fig attached 2408KB]