棉 花 学 报 Cotton Science 2006，18(4)：200-203
BAC Library Construction and Characterization of Suyuan 7235，A Cotton Germplasm with High Fiber Strength Abstracts：In this study，bacterial artificial chromosome (BAC) library of Suyuan7235，a cotton germplasm with high fiber strength has been constructed following the partial digestion of genomic DNA with Hind III. The pIndigoBAC-5( Hind III-cloning ready) cloning vector was used for the library. The BAC library included 30336 clones. Analysis of 96 recombinants showed that the insert DNA size ranged from 50 to 140 kb，averaged 120kb with less than 2.1% of empty clones. As much as 89.6% clones had inserts over 100 kb. As genomic resources，the library has potential use in further research of fiber strength gene analysis and gene cloning，as well as SSR primer development.
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