ÃÞ »¨ ѧ ±¨    Cotton Science   2010£¬22£¨5£©£º437-442

 

 

Study on Physiological and Biochemical Indicators of Seedling of Three New Seedling-raising and Transplanting Methods at Recovering Stage
LIU Xiao-ling, MAO Shu-chun*, HAN Ying-chun, LI Ya-bing, FAN Zheng-yi, WANG Guo-ping, FENG Lu, DONG Chun-wang, ZHU Qiao-ling
£¨Cotton Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetics Improvement, Ministry of Agriculture, Anyang, Henan 455000, China£©

Abstract: This study was conducted in spring and fall of 2009 to investigate three new seedling-raising methods (substrate seedling-raising and bare-root transplanting, floating seedling-nursing in nutrient water-bed and transplanting with substrate, as well as soilless seedling-raising and transplanting without carrier) through comparing the physiological and biochemical indicators of the seedlings at recovering stage. Seedlings were nursed in greenhouse. Experiment design was a randomized complete blocks in spring, and a micro-area design in autumn with three replications, respectively, in the field. The results showed that chlorophyll content, MDA content in seedlings of the three methods increased sharply due to the stress, and then decreased rapidly until a certain level and kept steady since then. Similarly, enzymatic activity of SOD, POD, CAT increased at the beginning and then decreased to a certain level and stayed stable. SOD activity changed relatively mildly comparing to that of POD and CAT, and enzymatic activity of the three methods showed a correlative relation. The dry weight, leaf area, and new root length increased along with the reproductive process. Moreover, after recovering stage, new roots of substrate seedling-raising method grew quickly and turned longer than that of soilless seedling-raising method, while less than that of floating seedling-raising method. But there were no significant difference in statistics. The dry weight and leaf area regarding seedlings of substrate seedling-raising method were between the other two methods.
Key words: cotton; new seedling-raising methods; recovering stage; physiology and biochemistry
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