棉 花 学 报    Cotton Science   2010，22（4）：360-366

Construction of Fingerprinting Map Based on SSR and Identification of Cultivars for Earliness Cultivars in Upland Cotton in Xinjiang
XUE Yan¹，ZHANG Xin-yu¹，SHA Hong³，LI Xue-yuan³，SUN Jie^1*，LI Bao-cheng^2*
(1.College of Agriculture / Key Laboratory of Oasis Eco-Agriculture of Bingtuan Production and Construction Group,Shihezi University, Shihezi，Xinjiang 832003, China; 2. Xinjiang Academy of Farmland Reclamation, Shihezi ，Xinjiang 832003, China; 3.Institute of Industrial Crops, Xinjiang Academy of Agricultural Science, Urumqi 830052, China)

Abstract：The research on 42 earliness cultivars planted in Xinjiang was conducted, using SSR molecular markers. Among 2300 pairs of primers, 52 pairs with steady polymorphism were selected. Three to 24 polymorphic loci were detected by each pair of primers. There were 506 polymorphic fragments detected in total. The average number of polymorphic fragments detected were 9.7 per pair of primers. The band size ranged from 100 to 2000 bp. The 52 SSR fingerprint maps were combined and analyzed comprehensively, it turned out to be that all the cotton cultivars could be identified by two primer pairs. The results indicated that the SSR markers can help to construct fingerprint map with high efficiency and accuracy rapidly, and it is feasible and effective to identify cotton cultivars with SSR molecular markers. This study will lay a solid foundation for rapid authentication and quality control of cotton seeds with large sample size.
Key words: upland cotton; SSR fingerprint; identification    [Full Text, 2502KB]