Ẵ »¨ ѧ ±¨ Cotton Science 2010£¬22£¨3£©£º285-288
Isolation and Analysis of Salt Tolerance Related Gene( GhVP ) from Gossypium hirsutum L. Abstract: Vacuolar proton-translocating inorganic pyrophosphatase acidify the vacuoles and power the vacuolar secondary active transport systems in plants. In the study, a gene, named GhVP , was isolated firstly by Rapid Amplification of cDNA End £¨RACE£© from the EST sequences of the Suppression Subtractive Hybridization £¨SSH£© library. Analysis of bioinformatic showed that the gene contains a uninterrupted open reading frame of 2301 bp, encoding for a polypeptide of 766 amino acids. Homology alignment revealed that the protein has higher homology with that from Arabidopsis thaliana, Nicotiana tobacum etc. The result of RT-PCR showed that the expression of GhVP varied under salt stress, which has the highest expression at 6 h after salt treatment and then decreased. The result was similar with that of Thellungiella halophila. Our study will contribute to understanding the salt-tolerance characteristic of glycophytic.
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