ÃÞ »¨ ѧ ±¨ Cotton Science 2010£¬22£¨3£©£º254-259
Comparison of Low Temperature-induced Protein in Cotton Seedlings with Different Chilling Resistance Abstract: By using two-dimensional electrophoresis and mass spectrometry techniques, the proteomes in different chilling-resistant levels cotton Jin3047 (It has obvious chilling resistance) and 410 (It has less chilling resistance) before and after low temperature treatments were comparatively analyzed. Results showed that the proteomes had a significant difference in different chilling-resistant level cotton varieties, within the range of pH4~7, there was a significant difference in the expression level of 50 protein spots in the protein profiles of cotton seedlings Jin3047 before and after low temperature treatment, and 30 of 50 protein spots were expressed. After low temperature treatment, the abundance value of 19 protein spots increased, with the absolute abundance being higher in Jin3047 than in 410, while that of 11 protein spots decreased, with the absolute abundance being lower in Jin3047 than in 410. The expression level of 8 protein spots in Jin3047 was characterized and the expression quantity was increasing, but no expression in 410. The differential protein spots were detected by the mass spectrometry, and identified by compared with the retrieval database. A total of 30 protein spots fingerprints were obtained, among which, 20% were of stress protein. These variously expressed proteins might play an important role in the chilling resistance of Jin3047.
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