ÃÞ »¨ ѧ ±¨    Cotton Science   2010£¬22£¨3£©£º242-247

 

 

Differences in SOD, POD, CAT Activities and MDA Content and their Responses to High Temperature Stress at Peak Flowering Stage in Cotton Lines with Different Tolerance to High Temperature
DENG Jiang-ming1, XIONG Ge-sheng2, YUAN Xiao-ling3, JIA Fei1, LIU Zhi1*
(1.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China; 2. Normal College of Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China; 3. Hunan Jin-nong Seed Co Ltd., Changsha, Hunan 410128, China)

Abstract:Activities of SOD, POD, CAT protective enzymes and MDA content in main-stem leaf and anther at different developmental stages and their responses to high temperature stress at peak flowering stage were analyzed comparatively using three cotton lines exhibiting tolerance, moderate sensitivity and sensitivity to high temperature in the present paper. The protective enzyme activities in main-stem leaves were nearly same among the three cotton lines at seedling and peak squaring stages, but significantly higher in tolerant line than susceptible ones at peak flowering and boll-setting stages. The MDA content was markedly lower in tolerant line than susceptible ones from peak squaring stage. Activities of protective enzymes in cotton anther increased with developmental processing, and remarkably higher SOD and POD activities in anther at the pollen maturing stage were measured in tolerant line. During the whole developmental period of anther, CAT activity was greater, while the MDA content was lower distinctly in tolerant line than susceptible ones. The protective enzyme activities were inhibited, and the MDA content increased excessively in main-stem leaves and anther of the three cotton lines under high temperature stress at the peak flowering stage. However, changes of decreasing in activities of protective enzymes and increasing in the MDA content in leaves and anther were less obviously in tolerant line than susceptible ones.
Key words: cotton£»high temperature tolerance£»SOD£»POD£»CAT£»MDA    [Full Text, 357KB]