棉 花 学 报    Cotton Science   2010，22（3）：195-201

Cloning of Stress-related Transcription Factors Gene from Cotton by Optimized TAIL-PCR
LIANG Cheng-zhen, ZHANG Rui, SUN Guo-qing, MENG Zhi-gang, ZHOU Tao, DING Zhong-tao, GUO San-dui*
(Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/ National Key Facility for Crop Gene Resources and Genetic Improvement,Beijing 100081, China)

Abstract: ABF/AREB/ABI5/DPBF transcription factors belong to basic leucine zipper protein, this kind of transcription factors plays an important role in the signal transduction pathways of ABA-dependent abiotic stress. In this study, taking structure characteristics of ABF/AREB/ABI5/DPBF as the research object, we cloned three bZIP encoding genes by 3′ RACE successfully from cotton and also optimized the TAIL-PCR conditions. The further experiment results showed that we had got the integrated sequences of the ORF, 3′UTR, as well as part of 5′UTR sequence. The genes we cloned will provide candidate genes for resistance genetic engineering of cotton. Furthermore, the optimized methodology of TAIL-PCR will provide an easy and efficient technology for cloning of target genes.
Key words: cotton; ABF/AREB/ABI5/DPBF; transcription factor; TAIL-PCR; stress-related genes
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