ÃÞ »¨ ѧ ±¨ Cotton Science 2010£¬22£¨2£©£º157-162
Cloning of Helicoverpa armigera Gene HaHR3 and Construction of Its RNA Interference Vector Abstract:The molt-regulating transcription factor (hormone receptor 3, HR3) controls the expression of related gene clusters in the process of molting and plays a key role in the regulation of molt cascade reactions. The cDNA segments of HaHR3, HR3 from Helicoverpa armigera , were cloned using RT-PCR. With an open reading frame of 1671 bp, HaHR3 showed 98% identity with HHR3 (AF337637) in homology comparison. The structure analysis indicated that HaHR3 has the typical characters of the superfamily of molt-regulating transcription factors, including two zinc finger motifs and a DNA banding domain. Neither signal peptide sequence nor N-glycosylated site was found in the HaHR3. Based on the sequence of HaHR3, the RNAi vector pRNAi1017-HaHR3sa was constructed. By subcloning the sense-antisense sequence of HaHR3 into the plant expression vector pCAMBIA2300-35S-OCS, pCAM-RNAi-HaHR3, the RNAi vector of the HaHR3 gene which is driven by the 35 Spromoter, was constructed. This provides a solid foundation for the application of the plant-mediated RNAi technique in the biological control of Helicoverpa armigera.
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