ÃÞ »¨ ѧ ±¨ Cotton Science 2010£¬22£¨2£©£º115-119
Molecular Variation of the Colored Cotton Lines Based on SSR Markers Abstract: In the study, SSR molecular marker was used to assess the molecular variation of 62 new lines of colored cottons from 12 combinations. The results showed that 29 of 242 pairs of SSR primers could generate polymorphic markers among the tested colored cotton lines. The total number of markers was 195, and the polymorphic markers were 115, accounted for 58.1%. MGHES-6 primer had the most polymorphic markers, followed by BNL2960, CM43 and MGHES-66. These primers could detect the variable SSR sites of the tested cottons very well. The PIC values for SSR primers varied from 0.493~0.938, averaged 0.790. The higher PIC values showed the abundant SSR alleles from the colored cottons. According to the SSR markers, 62 colored cottons were divided into two groups based on UPGMA method. One group included all the lines bred by pedigree selection, the other included the lines bred from hybridization method. The above results laid a foundation for breeding and germplasm utilization of colored cotton.
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