棉 花 学 报     Cotton Science    2010，22（2）：110-114

Isolation and Analysis of Drought-related Gene from Cotton ( Gossypium arboreum L.) SSH Library
ZHANG Ling, LI Fu-guang*, LIU Chuan-liang, ZHANG Chao-jun, WU Zhi-xia
（Cotton Research Institute，Chinese Academy of Agriculture Sciences；Key Laboratory of Cotton Genetic Improvement，Ministry of Agriculture，Anyang，Henan 455000, China）

Abstract：This study constructed high quality drought-related cDNA library of cotton ( Gossypium arboreum L.) by SSH successfully; it was checked and analyzed by Blue-white bolting, colony PCR, and bioinformatics. Finally, 392 positive clones were obtained. After cluster analysis of the ESTs sequences, 265 unigenes were obtained, including 41 contigs and 224 singlets. A large group of drought stress-induced genes were found in the cDNA library, which involved in many metabolism pathways.
Key words: Gossypium arboreum L; drought stress; SSH     [Full Text, 645KB]