ÃÞ »¨ ѧ ±¨ Cotton Science 2010£¬22£¨2£©£º104-109
Genetic Relationship and Diversity of the Germplasms in Gossypium barbadense L. from Four Different Countries Using SSR Markers
Abstract: Gemomic DNA of 20 germplasms of sea island cotton (G.barbadense L.) from four production countries(regions), namely former USSR, China, USA, and Egypt, were analyzed for their genetic relationship and genetic diversity, using the 3-78 £¨the standard line of G.barbadense£© as check and TM-1 (the standard line of G.hirsutum ) as reference check. The SSR molecular markers were employed and 108 pairs of polymorphic SSR primers were used in this experiment. Among the 108 pairs of polymorphic SSR primers, 175 polymorphic bands were produced, with averaging of 1.62 bands per primer. The genetic similarity coefficient among the tested germplasms was 0.66-0.94, with the average of 0.81. The UPGMA cluster analysis showed that four groups could be clearly clustered when genetic similarity coefficient was given as 0.77. The first group were those from former USSR, most germplasms in the second group came from USA, all of the germplasms in the third group came from China, and most in the last group were those from Egypt. The results showed that the genetic diversity of the sea-island cotton germpalsms from USSR was extraordinary diversified among the four different countries, and that from Egypt was the least in genetic diversity. The sea-island cotton germplasms from China were placed in the middle, according to the results of genetic similarity coefficient in present experiment. Our results also showed that the genetic background of the sea-island cotton germplasms tested in this experiment was correlative with their producing regions, and the SSR marker could be used in revealing the genetic diversity and genetic relationship among the sea-island germplasms.
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