ÃÞ »¨ ѧ ±¨ Cotton Science 2010£¬22£¨2£©£º99-103
Molecular Cloning and Characterization of GhDIS2 in Cotton Abstract: Cotton fibers are single-celled seed trichomes of major economic importance. Many important genes are expressed during cotton fiber development. DISTORTED2 encodes the Arabidopsis homolog of the ARPC2 subunit of the ARP2/3 complex, which binds to the sides of existing actin filaments and efficiently nucleates new filaments. The cytoskeleton arrangement processes in the cotton fiber initial and elongation. In this study, the Gossypium homology of the ARPC2 subunit named as GhDIS2 was isolated from the TM-1 ovule on the day of flowering. GhDIS2 contained 975 bp open reading frame, encoding a polypeptide containing 324 amino acids. GhDIS2 was expressed constitutively in every tissue with different expression levels, with the peak in 12 days post anthesis (DPA) fiber cells and low expression levels in 18, 24 DPA fiber cells, roots and stems. Southern blotting showed that GhDIS2 had two copies in allotetraploid cotton genome. A shuttle vector contained GhDIS2 cDNA sequence was transformed into fission yeast. GhDIS2 led to a significant increase in cell length and width. These results indicated that GhDIS2 had the same function to DIS in other plants.
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