ÃÞ »¨ ѧ ±¨     Cotton   Science   2010£¬22£¨1£©£º42-48

 

 

Cloning and Quantitative Analysis by Real-Time RT-PCR of a Chalcones Synthase Gene £¨GhCHS1£© in the Natural Brown Cotton Fibers
YANG Hui-na, TIAN Xin-hui, LI Yan-jun, LI Ming-yue, FENG Hong-jie, SUN Jie*
(College of Agriculture/The Key Oasis Eco-agriculture Laboratory of Xinjiang Production and Construction Group, Shihezi University, Shihezi, Xinjiang 832003, China)

Abstract: A full-length cDNA encoding CHS was cloned from brown cotton fiber of 18 days post anthesis by RT-PCR and rapid amplification of cDNA ends (RACE) using specific primers based on the highly conserved sequences of plant CHS that had already known. Blast search revealed that it is a new gene, and was named as GhCHS 1 (GenBank accession: EU921263). The temporal and spatial expression patterns of GhCHS 1 gene were examined by the real-time quantitative PCR with the specific primers. The expression analysis indicated that GhCHS 1 gene was preferentially expressed in brown cotton fibers during development, and the expressed levels of GhCHS 1 gene in white fibers and green fibers were very low or undetectable, which suggested that GhCHS 1 gene might play an important role in brown pigmentation of naturally brown cotton fibers.
Key words: chalcones synthase; natural colored cotton; real-time quantitative PCR
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