棉 花 学 报     Cotton   Science 2010，22（1）：36-41

Application of SNAC1 Gene as a Selection Marker Gene for Cotton （Gossypium hirsutum L.）Transformation
LI Xue-lin^1,2, LIU Guan-ze¹, NIE Yi-chun¹, GUO Xiao-ping¹, ZHANG Xian-long^1*
(1.National Key Lab of Crop Genetic and Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2.College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471003, China)

Abstract: Employing SNAC1 gene as a selection marker gene and NaCl as a selection agent, SNAC1 and GUS genes were introduced into cotton genome via Agrobacterium. Transgenic calli were confirmed by PCR analysis, and expression of the GUS gene was showed with GUS staining. The concentration and method for employing NaCl as a selection agent had been studied. The reasonable selection concentration of NaCl should be 1.1%-1.5%(W/V). The start concentration of NaCl in callus induction medium should be lower and the concentration increased as calli proliferated. Because NaCl is not beneficial for embryo differentiation and development, NaCl should be removed from the culture medium after the calli were subcultured for 2-3 times.
Key words: cotton; transformation; SNAC1 gene; NaCl     [Full Text, 665KB]