ÃÞ »¨ ѧ ±¨ Cotton Science 2010£¬22£¨1£©£º30-35
Molecular Cloning and Tissue Expression of Two Cytochrome P450 cDNA Fragments in the Antennae of Male Spodoptera exigua (H¨¹bner) Abstract: By reverse transcription-polymerase chain reaction (RT-PCR) method, two cDNA fragments of P450 genes CYP 4L 15 and CYP 4L 16 were cloned using a pair of degenerate primers. The two fragments had the conserved I-helix and heme-binding domains of P450, and shared high identities with several putative insect odorant-degrading P450s. Further tissue expression analysis of the two genes showed that two genes might function as odorant-degrading enzymes, as they were highly expressed in antennae. However, their low expression in head and thorax for both genes, as well as in abdomen for CYP 4L 15 suggested that these genes had other functions besides the odorant degradation.
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