ÃÞ »¨ ѧ ±¨       Cotton Science       2009£¬21(4)£º330-334

 

 

 

Research Progress of Molecular Marker Technology Applied in Cotton Variety Identification
KUANG Meng£¬YANG Wei-hua£¬XU Hong-xia£¬WANG Yan-qin£¬ZHOU Da-yun£¬FENG Xin-ai£¬WANG Jun-fang
£¨Cotton Research Institute, CAAS; Supervision, Inspection and Test Center of Cotton Quality, Ministry of Agriculture,Anyang, Henan 455000£¬China£©

Abstract£ºThere are three phases of DNA fingerprint technology based on molecular marker£ºthe first is RFLP based on Southern blot£¬the second are several markers based on PCR and the third is SNP£¨single nucleotide polymorphism £©.Research and application of variety identification technology was discussed£¬four main molecular marker technologies applied in cotton variety identification were introduced in detail as follow:RFLP,RAPD,AFLP and SSR.Technology principle,research and application status,advantage and disadvantage about each molecular marker applied in cotton variety identification were expatiated.As compared£¬the viewpoint was put forward:SSR marker was the most appropriate molecular marker in cotton variety identification,and the necessity of construction of Chinese cotton DNA fingerprint database based on SSR marker technology was analyzed.
Key words£ºcotton£»molecular marker£»variety identification£»DNA fingerprint database
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